通过研究不同光照处理下红花龙胆代谢物变化,分析其主要代谢路径,并筛选参与光照胁迫的关键差异代谢物。结果表明,共检测到1298个代谢物,其中MS45 vs MS0中共检测到196个差异表达成分,53个表达上调,143个表达下调;MS90 vs MS0中检测到16...通过研究不同光照处理下红花龙胆代谢物变化,分析其主要代谢路径,并筛选参与光照胁迫的关键差异代谢物。结果表明,共检测到1298个代谢物,其中MS45 vs MS0中共检测到196个差异表达成分,53个表达上调,143个表达下调;MS90 vs MS0中检测到165个差异表达成分,59个表达上调,106个表达下调;MS90 vs MS45中共检测到90个差异表达成分,57个表达上调,33个表达下调。富集分析显示,三个比较组共经过6条相同的富集通路,4条显著富集。黄酮类化合物占所检测到总代谢物的48%,黄酮和黄酮醇类占黄酮类化合物的67.4%,黄酮和黄酮醇类是光照胁迫响应中的重要差异代谢物;KEGG富集分析表明,不同光照处理的红花龙胆代谢物数量差异显著,主要受黄酮和黄酮醇类向上积累模式的影响,是参与红花龙胆对强光照胁迫响应的关键物质。代谢组学方法能够快速且有效地分析不同遮荫处理的红花龙胆体内代谢物变化,其代谢物在一定程度上能反映红花龙胆在不同光照处理代谢物变化的规律和生理生态响应。展开更多
OBJECTIVE This work aimed to investigate the anti-rheumatoid arthritic effect of gentio.picroside from Gentiana macrophylla Pall using an animal model of adjuvant induced arthritis.METH.ODS Adjuvant arthritis was indu...OBJECTIVE This work aimed to investigate the anti-rheumatoid arthritic effect of gentio.picroside from Gentiana macrophylla Pall using an animal model of adjuvant induced arthritis.METH.ODS Adjuvant arthritis was induced in fifty SD male rats,which were randomly divided into five groups(n=10):control(0.5% CMC-Na) group,AIA(rats with CFA) group,dexamethasone(1 mg·kg^(-1)) group,gentiopicroside(50 mg·kg^(-1)) group,and gentiopicroside(100 mg·kg^(-1)) group.Rats were administered intragastrically with drugs or CMC-Na once a day for a period of 2 weeks.Paw swelling,arthritic index,histological changes were assessed to evaluate the anti-arthritic effect.Weight growth,spleen and thymus indexes were also investigated in.RESULTS Gentiopicroside at dose of 100 mg·kg^(-1) significantly inhibited the secondary paw swelling(P<0.05) and arthritis index(P<0.05),decreased synovial inflammatory infil.tration,synovial hyperplasia and bone erosion.Furthermore,gentiopicroside showed no immunosup.pressive adverse effects in body weight,index of spleen and thyums compared with dexamethasone administration(P<0.05,P<0.01).CONCLUSION Gentiopicroside possessed anti-arthritic efficacy in AIA rats without immunosuppressive effects.展开更多
文摘通过研究不同光照处理下红花龙胆代谢物变化,分析其主要代谢路径,并筛选参与光照胁迫的关键差异代谢物。结果表明,共检测到1298个代谢物,其中MS45 vs MS0中共检测到196个差异表达成分,53个表达上调,143个表达下调;MS90 vs MS0中检测到165个差异表达成分,59个表达上调,106个表达下调;MS90 vs MS45中共检测到90个差异表达成分,57个表达上调,33个表达下调。富集分析显示,三个比较组共经过6条相同的富集通路,4条显著富集。黄酮类化合物占所检测到总代谢物的48%,黄酮和黄酮醇类占黄酮类化合物的67.4%,黄酮和黄酮醇类是光照胁迫响应中的重要差异代谢物;KEGG富集分析表明,不同光照处理的红花龙胆代谢物数量差异显著,主要受黄酮和黄酮醇类向上积累模式的影响,是参与红花龙胆对强光照胁迫响应的关键物质。代谢组学方法能够快速且有效地分析不同遮荫处理的红花龙胆体内代谢物变化,其代谢物在一定程度上能反映红花龙胆在不同光照处理代谢物变化的规律和生理生态响应。
文摘OBJECTIVE This work aimed to investigate the anti-rheumatoid arthritic effect of gentio.picroside from Gentiana macrophylla Pall using an animal model of adjuvant induced arthritis.METH.ODS Adjuvant arthritis was induced in fifty SD male rats,which were randomly divided into five groups(n=10):control(0.5% CMC-Na) group,AIA(rats with CFA) group,dexamethasone(1 mg·kg^(-1)) group,gentiopicroside(50 mg·kg^(-1)) group,and gentiopicroside(100 mg·kg^(-1)) group.Rats were administered intragastrically with drugs or CMC-Na once a day for a period of 2 weeks.Paw swelling,arthritic index,histological changes were assessed to evaluate the anti-arthritic effect.Weight growth,spleen and thymus indexes were also investigated in.RESULTS Gentiopicroside at dose of 100 mg·kg^(-1) significantly inhibited the secondary paw swelling(P<0.05) and arthritis index(P<0.05),decreased synovial inflammatory infil.tration,synovial hyperplasia and bone erosion.Furthermore,gentiopicroside showed no immunosup.pressive adverse effects in body weight,index of spleen and thyums compared with dexamethasone administration(P<0.05,P<0.01).CONCLUSION Gentiopicroside possessed anti-arthritic efficacy in AIA rats without immunosuppressive effects.
