摘要
目的建立五岭龙胆的鉴别研究方法。方法采用生药学常规方法,对五岭龙胆进行原植物和药材性状观察,并对其鉴别特征进行描述;通过常规光学显微镜、扫描电镜等技术结合多种显微制片方法对五岭龙胆进行不同组织部位及全草粉末的研究。结果五岭龙胆原植物、性状和显微组织构造等特征明显;五岭龙胆根横切面可见后生皮层,茎横切面凯氏带明显。叶上表皮可见头状凸起,表皮细胞壁呈浅波状弯曲;叶下表皮可见气孔和非腺毛,表皮细胞壁呈深波状弯曲。花瓣上表皮具有排列规律的脑纹状类球形突起,基部呈流苏状;花瓣下表皮可见排列规律的脊状扁长条形隆起。花粉粒呈长圆形,具有三条萌发沟,表面饰纹网眼大小不一。种子呈梨形,表面蜂窝状,可见Ⅰ和Ⅱ饰纹。全草粉末特征可见外皮层细胞等显微特征。结论五岭龙胆的形态组织特点为其准确鉴定及应用提供科学依据。
Objective To establish an identification method for Gentiana davidii Franch.Methods Original plants and herbal properties were identified with pharmacognostical method,and its diagnostic characteristics were described.Different tissue parts and whole plant powder of G.davidii Franch.were studied by conventional optical microscopy,scanning electron microscopy combined with various microscopy methods.Results The morphological,trait and microstructure characteristics of G.davidii Franch.were obvious.The microscopic characteristics of the tissues were metazoan cortex visible in the transverse section of the roots,and the casparian strip was obvious in the transverse section of the stems.The epidermis of the leaves showed a cephalic bulge,and the cell wall of the epidermis cells showed a shallow wavy curve.There were stomata and non-glandular hairs in the lower epidermis of the leaves,and the cell walls of the epidermis cells were deeply wavy.The upper epidermis of the petal has a regular arrangement of cerebral striatum spherical protrusions,and the lower epidermis has a regular arrangement of ridge flat elongated protrusions.The pollen grains were oblong with three germination furrows and different sizes of surface decorative mesh.The seed was pear-shaped,the surface was honeycomb,I and II could be seen.Conclusion The microstructure of the features tissue of G.davidii Franch.can provide a basis for its authenticity identification,quality evaluation and formulation of quality standards,and at the same time offer e a new taxonomic basis for the taxonomy of gentian plants.
作者
彭江丽
彭兰淳
王鹏
彭求贤
郑青竹
李顺祥
PENG Jiangli;PENG Lanchun;WANG Peng;PENH Qiuxian;ZHEN Qingzhu;LI Shunxiang(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine,Changsha 410208,China;Hunan Province Sino-US International Joint Research Center for Therapeutic Drugs of Senile Degenerative Diseases,Changsha 410208,China)
出处
《世界科学技术-中医药现代化》
北大核心
2025年第2期375-383,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上项目(81973593):miRNA-506调控cav-1/PI3K/Akt通路在尖尾芋凝集素抑制乳腺癌中的作用,负责人:彭求贤
湖南省自然科学基金科药联合项目(2022JJ80087):基于NF-κB/Nrf2通路交互作用研究五岭龙胆乙酸乙酯提取物对小鼠急性肝损伤的保护及作用机制,负责人:彭江丽。
关键词
五岭龙胆
显微特征
形态组织学
扫描电镜
Gentiana davidi Franch.
Microscopic characteristics
Morphologic histology
Scanning electron microscopy
作者简介
通讯作者:李顺祥(ORCID:0000-0001-9866-6609),主要研究方向:从事中药药效物质基础与作用机制研究。
