Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interacti...Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interaction dynamics between the cotton plant host and V.dahliae pathogen pose a crucial predicament for effectively managing cotton Verticillium wilt.Nevertheless,the most cost-effective approach to controlling this disease involves breeding and cul-tivating resistant cotton varieties,demanding a meticulous analysis of the mechanisms underlying cotton’s resistance to Verticillium wilt and the identification of pivotal genes.These aspects constitute focal points in disease-resistance breeding programs.In this review,we comprehensively discuss genetic inheritance associated with Verticillium wilt resistance in cotton,the advancements in molecular markers for disease resistance,the functional investiga-tion of resistance genes in cotton,the analysis of pathogenicity genes in V.dahliae,as well as the intricate interplay between cotton and this fungus.Moreover,we delve into the future prospects of cutting-edge research on cotton Verticillium wilt,aiming to proffer valuable insights for the effective management of this devastating fungus.展开更多
Soybean frogeye leaf spot(FLS)disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed qual...Soybean frogeye leaf spot(FLS)disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed quality of soybean.The current resistant varieties are difficult to meet the production demand.The breeders have identified 50 different physiological small species and discussed the physiological and biochemical characteristics of soybean resistance to FLS.In soybean disease resistance breeding,resistance resources are screened for the main physiological races in different countries,resistance materials are created,more than 100 genome regions associated with resistance are located,and 12 resistance-related genes are identified.In order to promote the research of soybean disease resistance breeding,this paper expounded and analyzed the pathogenesis characteristics of soybean FLS,the division of races,the physiological and biochemical mechanism of soybean resistance to FLS disease,quantitative trait locus(QTL),quantitative trait nucleotides(QTN),genes of resistance sites,the screening of resistant germplasm resources,and the breeding of new varieties,so as to gain an in-depth understanding of the pathogenesis principle of soybean FLS disease.In order to provide a theoretical basis and technical support for the breeding of soybean FLS disease,the resistance mechanism of soybean FLS disease was analyzed from the molecular level.展开更多
Curcumin(Cur)is an important bioactive component of polyphenols in the rhizomes of Curcuma longa L.,Tulipa gesneriana L.and other Curcuma plants.It has a wide range of pharmacological effects such as anti-tumor,anti-a...Curcumin(Cur)is an important bioactive component of polyphenols in the rhizomes of Curcuma longa L.,Tulipa gesneriana L.and other Curcuma plants.It has a wide range of pharmacological effects such as anti-tumor,anti-atherosclerosis,anti-inflammatory,and neuroprotection.Parkinson disease(PD)is a neurodegenerative disease that often occurs in the elderly.Its main pathological characteristics are the characteristic loss of substantia nigra dopaminergic neurons,the decrease of dopamine content in the striatum,and the formation of Lewy bodies.At present,the main methods of clinical treatment of PD include drug therapy and surgical operation,but due to its complicated pathogenesis,they can only play a role in relieving,but cannot be completely cured.Modern pharmacological studies have shown that Cur has certain effects in the treatment of PD.①Anti-oxidative stress:oxidative stress is closely related to the degeneration of dopaminergic neurons.Studies have found that Cur can increase the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),reduce malondialdehyde(MDA)content,thereby reducing oxidative stress damage and protecting dopaminergic neuron.②Reduce inflammation in brain tissue:neuroinflammation plays an important role in the development of PD.Reducing the level of inflammatory factors can have a certain therapeutic effect on PD.Studies have shown that high-dose Cur can reduce the levels of interleukin-6(IL-6),IL^(-1)β,and tumor necrosis factor-α(TNF-α)in brain tissue,reduce inflammation,inhibit further neuronal damage,improve learning and memory,and exert neuroprotective effects.③Activation of autophagy:the abnormal accumulation ofα-Synuclein(α-Syn)in Lewy bodies is closely related to PD,and autophagy dysfunction leads toα-Syn clearance obstacles and an important factor of abnormal aggregation.Cur can increase the expression of microtubule-associated protein 1 light chain 3(LC3-Ⅱ)and lysosome-associated membrane protein 2A(LAMP2A),and reduce the protein and mRNA expression ofα-Syn.It can be seen that Cur promotes the elimination ofα-Syn and protects neurons from damage by activating autophagy.④Inhibition of mitochondrial dysfunction:mitochondria plays a central regulatory role in the process of cell apoptosis,and mitochondrial dysfunction is related to reactive oxygen species,energy and mitochondrial membrane potential,which may cause substantia nigra striatal neuropathy.Experiments have shown that Cur can reduce the active oxygen content in PC12 cells induced by MPP+,maintain the normal membrane potential of mitochondria,thereby stabilizing mitochondrial function and inhibiting PC12 cell apoptosis.This study summarized the action mechanism of Cur in the treatment of PD,and clarified the basis of its pharmacodynamics, providing a reference for the clinical research and new drug develop ment research of Cur in the treatment of PD.展开更多
OBJECTIVE To explore the new indications and key mechanism of Bazi Bushen capsule(BZBS)by network pharmacology and in vitro experiment.METHODS The potential tar⁃get profiles of the components of BZBS were pre⁃dicted.S...OBJECTIVE To explore the new indications and key mechanism of Bazi Bushen capsule(BZBS)by network pharmacology and in vitro experiment.METHODS The potential tar⁃get profiles of the components of BZBS were pre⁃dicted.Subsequently,new indications for BZBS were predicted by disease ontology(DO)enrich⁃ment analysis and initially validated by GO and KEGG pathway enrichment analysis.Further⁃more,the therapeutic target of BZBS acting on AD signaling pathway were identified by intersec⁃tion analysis.Two Alzheimer′s disease(AD)cell models,BV-2 and SH-SY5Y,were used to pre⁃liminarily verify the anti-AD efficacy and mecha⁃nism of BZBS in vitro.RESULTS In total,1499 non-repeated ingredients were obtained from 16 herbs in BZBS formula,and 1320 BZBS targets with high confidence were predicted.Disease enrichment results strongly suggested that BZBS formula has the potential to be used in the treat⁃ment of AD.In vitro experiments showed that BZ⁃BS could significantly reduce the release of TNF-αand IL-6 and the expression of COX-2 and PSEN1 in Aβ25-35-induced BV-2 cells.BZBS reduced the apoptosis rate of Aβ25-35 induced SH-SY5Y cells,significantly increased mitochon⁃drial membrane potential,reduced the expres⁃sion of Caspase3 active fragment and PSEN1,and increased the expression of IDE.CONCLU⁃SIONS BZBS formula has a potential use in the treatment of AD,which is achieved through regu⁃lation of ERK1/2,NF-κB signaling pathways,and GSK-3β/β-catenin signaling pathway.Further⁃more,the network pharmacology technology is a feasible drug repurposing strategy to reposition new clinical use of approved TCM and explore the mechanism of action.The study lays a foun⁃dation for the subsequent in-depth study of BZBS in the treatment of AD and provides a basis for its application in the clinical treatment of AD.展开更多
This review has looked back to applying studies of Berberine in treatment for congestive heart failure,cardiac arrhythmias,hypertension and ischemic heart disease in recent years.It has summarized that the mechanism a...This review has looked back to applying studies of Berberine in treatment for congestive heart failure,cardiac arrhythmias,hypertension and ischemic heart disease in recent years.It has summarized that the mechanism and clinical progress of Berberine in treatment for cardiovascular disease.The viewpoint has been proposed the Berberine may be a benefit agent in therapy of cardiovascular disease.展开更多
淡漠是脑小血管病(cerebral small vessel disease,CSVD)常见的神经精神症状,临床常表现为目标导向行为减少、认知活动降低及情感表达减少等。CSVD患者淡漠的出现可能与神经网络功能障碍有关,且与认知功能损害存在密切联系。本文旨在对C...淡漠是脑小血管病(cerebral small vessel disease,CSVD)常见的神经精神症状,临床常表现为目标导向行为减少、认知活动降低及情感表达减少等。CSVD患者淡漠的出现可能与神经网络功能障碍有关,且与认知功能损害存在密切联系。本文旨在对CSVD患者淡漠的神经网络机制进行综述。展开更多
基金supported by National Natural Science Foundation of China(32201752)Xinjiang Tianchi Talents Program (TCYC2023TP02)Key Project of the Natural Science Foundation of Xinjiang Production and Construction Corps (2024DA001)
文摘Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interaction dynamics between the cotton plant host and V.dahliae pathogen pose a crucial predicament for effectively managing cotton Verticillium wilt.Nevertheless,the most cost-effective approach to controlling this disease involves breeding and cul-tivating resistant cotton varieties,demanding a meticulous analysis of the mechanisms underlying cotton’s resistance to Verticillium wilt and the identification of pivotal genes.These aspects constitute focal points in disease-resistance breeding programs.In this review,we comprehensively discuss genetic inheritance associated with Verticillium wilt resistance in cotton,the advancements in molecular markers for disease resistance,the functional investiga-tion of resistance genes in cotton,the analysis of pathogenicity genes in V.dahliae,as well as the intricate interplay between cotton and this fungus.Moreover,we delve into the future prospects of cutting-edge research on cotton Verticillium wilt,aiming to proffer valuable insights for the effective management of this devastating fungus.
基金Supported by the 14th Five-Year National Key Research and Development Program(2021YFD1201103–01–05)the National Natural Science Foundation of China(32301819)the Cooperation Project of Research and Development Center between Wudalianchi Government and Northeast Agricultural University.
文摘Soybean frogeye leaf spot(FLS)disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed quality of soybean.The current resistant varieties are difficult to meet the production demand.The breeders have identified 50 different physiological small species and discussed the physiological and biochemical characteristics of soybean resistance to FLS.In soybean disease resistance breeding,resistance resources are screened for the main physiological races in different countries,resistance materials are created,more than 100 genome regions associated with resistance are located,and 12 resistance-related genes are identified.In order to promote the research of soybean disease resistance breeding,this paper expounded and analyzed the pathogenesis characteristics of soybean FLS,the division of races,the physiological and biochemical mechanism of soybean resistance to FLS disease,quantitative trait locus(QTL),quantitative trait nucleotides(QTN),genes of resistance sites,the screening of resistant germplasm resources,and the breeding of new varieties,so as to gain an in-depth understanding of the pathogenesis principle of soybean FLS disease.In order to provide a theoretical basis and technical support for the breeding of soybean FLS disease,the resistance mechanism of soybean FLS disease was analyzed from the molecular level.
基金Key Research and Development Program of Hebei Province(20370509D)and Science and Technology Project of Hebei Education Department(ZD2020117)。
文摘Curcumin(Cur)is an important bioactive component of polyphenols in the rhizomes of Curcuma longa L.,Tulipa gesneriana L.and other Curcuma plants.It has a wide range of pharmacological effects such as anti-tumor,anti-atherosclerosis,anti-inflammatory,and neuroprotection.Parkinson disease(PD)is a neurodegenerative disease that often occurs in the elderly.Its main pathological characteristics are the characteristic loss of substantia nigra dopaminergic neurons,the decrease of dopamine content in the striatum,and the formation of Lewy bodies.At present,the main methods of clinical treatment of PD include drug therapy and surgical operation,but due to its complicated pathogenesis,they can only play a role in relieving,but cannot be completely cured.Modern pharmacological studies have shown that Cur has certain effects in the treatment of PD.①Anti-oxidative stress:oxidative stress is closely related to the degeneration of dopaminergic neurons.Studies have found that Cur can increase the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),reduce malondialdehyde(MDA)content,thereby reducing oxidative stress damage and protecting dopaminergic neuron.②Reduce inflammation in brain tissue:neuroinflammation plays an important role in the development of PD.Reducing the level of inflammatory factors can have a certain therapeutic effect on PD.Studies have shown that high-dose Cur can reduce the levels of interleukin-6(IL-6),IL^(-1)β,and tumor necrosis factor-α(TNF-α)in brain tissue,reduce inflammation,inhibit further neuronal damage,improve learning and memory,and exert neuroprotective effects.③Activation of autophagy:the abnormal accumulation ofα-Synuclein(α-Syn)in Lewy bodies is closely related to PD,and autophagy dysfunction leads toα-Syn clearance obstacles and an important factor of abnormal aggregation.Cur can increase the expression of microtubule-associated protein 1 light chain 3(LC3-Ⅱ)and lysosome-associated membrane protein 2A(LAMP2A),and reduce the protein and mRNA expression ofα-Syn.It can be seen that Cur promotes the elimination ofα-Syn and protects neurons from damage by activating autophagy.④Inhibition of mitochondrial dysfunction:mitochondria plays a central regulatory role in the process of cell apoptosis,and mitochondrial dysfunction is related to reactive oxygen species,energy and mitochondrial membrane potential,which may cause substantia nigra striatal neuropathy.Experiments have shown that Cur can reduce the active oxygen content in PC12 cells induced by MPP+,maintain the normal membrane potential of mitochondria,thereby stabilizing mitochondrial function and inhibiting PC12 cell apoptosis.This study summarized the action mechanism of Cur in the treatment of PD,and clarified the basis of its pharmacodynamics, providing a reference for the clinical research and new drug develop ment research of Cur in the treatment of PD.
基金Chinese Academy of Engi⁃neering Strategic Consulting Project(2022-XY-45)S&T Program of Hebei(22372502D)+1 种基金Scien⁃tific Research Project of Hebei Provincial Admin⁃istration of Traditional Chinese Medicine(023172)and Scientific Research Project of Hebei Provincial Administration of Traditional Chinese Medicine(2021273)。
文摘OBJECTIVE To explore the new indications and key mechanism of Bazi Bushen capsule(BZBS)by network pharmacology and in vitro experiment.METHODS The potential tar⁃get profiles of the components of BZBS were pre⁃dicted.Subsequently,new indications for BZBS were predicted by disease ontology(DO)enrich⁃ment analysis and initially validated by GO and KEGG pathway enrichment analysis.Further⁃more,the therapeutic target of BZBS acting on AD signaling pathway were identified by intersec⁃tion analysis.Two Alzheimer′s disease(AD)cell models,BV-2 and SH-SY5Y,were used to pre⁃liminarily verify the anti-AD efficacy and mecha⁃nism of BZBS in vitro.RESULTS In total,1499 non-repeated ingredients were obtained from 16 herbs in BZBS formula,and 1320 BZBS targets with high confidence were predicted.Disease enrichment results strongly suggested that BZBS formula has the potential to be used in the treat⁃ment of AD.In vitro experiments showed that BZ⁃BS could significantly reduce the release of TNF-αand IL-6 and the expression of COX-2 and PSEN1 in Aβ25-35-induced BV-2 cells.BZBS reduced the apoptosis rate of Aβ25-35 induced SH-SY5Y cells,significantly increased mitochon⁃drial membrane potential,reduced the expres⁃sion of Caspase3 active fragment and PSEN1,and increased the expression of IDE.CONCLU⁃SIONS BZBS formula has a potential use in the treatment of AD,which is achieved through regu⁃lation of ERK1/2,NF-κB signaling pathways,and GSK-3β/β-catenin signaling pathway.Further⁃more,the network pharmacology technology is a feasible drug repurposing strategy to reposition new clinical use of approved TCM and explore the mechanism of action.The study lays a foun⁃dation for the subsequent in-depth study of BZBS in the treatment of AD and provides a basis for its application in the clinical treatment of AD.
文摘This review has looked back to applying studies of Berberine in treatment for congestive heart failure,cardiac arrhythmias,hypertension and ischemic heart disease in recent years.It has summarized that the mechanism and clinical progress of Berberine in treatment for cardiovascular disease.The viewpoint has been proposed the Berberine may be a benefit agent in therapy of cardiovascular disease.
文摘淡漠是脑小血管病(cerebral small vessel disease,CSVD)常见的神经精神症状,临床常表现为目标导向行为减少、认知活动降低及情感表达减少等。CSVD患者淡漠的出现可能与神经网络功能障碍有关,且与认知功能损害存在密切联系。本文旨在对CSVD患者淡漠的神经网络机制进行综述。
