为了解华重楼根际芽胞杆菌种群多样性信息,本研究采用稀释平板法分离福建省光泽县华重楼品种根际的芽胞杆菌,并对其进行16S r RNA序列分析。结果表明:从华重楼根际共分离到17个形态差异的芽胞杆菌菌株;16S r RNA序列分析表明,17个菌株...为了解华重楼根际芽胞杆菌种群多样性信息,本研究采用稀释平板法分离福建省光泽县华重楼品种根际的芽胞杆菌,并对其进行16S r RNA序列分析。结果表明:从华重楼根际共分离到17个形态差异的芽胞杆菌菌株;16S r RNA序列分析表明,17个菌株鉴定为8个种,归属于4个属,芽胞杆菌属(Bacillus)、赖氨酸芽胞杆菌属(Lysinibacillus)、类芽孢杆菌属(Paenibacillus)和绿芽胞杆菌属(Viridibacillus),其中芽胞杆菌属的种类和数量最多。经抑菌试验分析,芽孢杆菌属的特基拉芽孢杆菌FJAT-43012对尖孢镰刀菌具有抑制作用。本研究结果表明,华重楼根际芽胞杆菌种群多样性较为丰富。本研究对芽胞杆菌资源的开发和利用提供良好的实验材料和理论基础。展开更多
Particle induced X- ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) have been used extensively for analytical purpose because of their quantitative accuracy, reliability, simplicity and capability...Particle induced X- ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) have been used extensively for analytical purpose because of their quantitative accuracy, reliability, simplicity and capability of non- destructive and multielement analysis. When these techniques are combined with a scanning microbeam system and a data acquisition system, three dimensional distribution of elemental composition can be displayed. Samples analyzed so far at Fudan University include a microelectronic circuit and some biological and archaeological samples. The PIXE and RBS spectra and the secondary electron images have been measured.展开更多
文摘为了解华重楼根际芽胞杆菌种群多样性信息,本研究采用稀释平板法分离福建省光泽县华重楼品种根际的芽胞杆菌,并对其进行16S r RNA序列分析。结果表明:从华重楼根际共分离到17个形态差异的芽胞杆菌菌株;16S r RNA序列分析表明,17个菌株鉴定为8个种,归属于4个属,芽胞杆菌属(Bacillus)、赖氨酸芽胞杆菌属(Lysinibacillus)、类芽孢杆菌属(Paenibacillus)和绿芽胞杆菌属(Viridibacillus),其中芽胞杆菌属的种类和数量最多。经抑菌试验分析,芽孢杆菌属的特基拉芽孢杆菌FJAT-43012对尖孢镰刀菌具有抑制作用。本研究结果表明,华重楼根际芽胞杆菌种群多样性较为丰富。本研究对芽胞杆菌资源的开发和利用提供良好的实验材料和理论基础。
文摘Particle induced X- ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) have been used extensively for analytical purpose because of their quantitative accuracy, reliability, simplicity and capability of non- destructive and multielement analysis. When these techniques are combined with a scanning microbeam system and a data acquisition system, three dimensional distribution of elemental composition can be displayed. Samples analyzed so far at Fudan University include a microelectronic circuit and some biological and archaeological samples. The PIXE and RBS spectra and the secondary electron images have been measured.
