摘要
对药用石斛进行组织培养及管理研究中发现 ,在附加了 2 0mg/LBA和 0 2mg/LIBA的改良N6培养基上 ,原球茎的诱导率可达 95 %以上 ;在分化增殖阶段 ,采用附加了 0 1mg/LBA ,5 %～ 10 %香蕉汁和 5 %～ 10 %土豆汁的改良N6培养基上 ,可增殖达 8～ 10倍 ;在生根壮苗阶段 ,采用附加了 10 %香蕉汁和 0 2mg/LNAA的改良N6的培养基上 ,效果最佳。移栽以后 ,对石斛的田间生长采用科学合理的管理方式 ,可使石斛的幼苗成活率大为提高。
The research of the tissue culture and management on medicial Dendorbium show that the induction frequency of protocorn-like body amounts to more than 95% on the modified N_6 medium with 2.0mg/L BA和0.2mg/L IBA.At the propagation-differentiation stage,it can propagate 8～10 times when using the modified N_6 medium supplemented with 0.1mg/L BA,5%～10%potato exerait and 5%～10% banana juice;At the plantlet-promoting stage,the effect of modified N_6 supplemented with 0.2mg/L NAA and 10% banana juice is the best .After being replanted, the survival rate of seedling improve highly when scientific reasonable management way being used in the field.
出处
《南阳师范学院学报》
CAS
2004年第6期41-44,共4页
Journal of Nanyang Normal University
基金
河南省科学技术厅科技攻关项目 ( 0 3 2 40 70 0 5 5 )
关键词
石斛
试管苗
组织培养
原球茎
Dendrobium
in vitrc seedlings
tissue culture
protocorn-like bodies
