摘要
目的 :探讨 10 -HCPT对人肝癌细胞QGY和HepG2生长增殖的影响。 方法 :分别用不同浓度 10 -HCPT作用QGY和HepG2细胞 4 8h和相同浓度作用不同时间 ,以MTT法检测细胞生长指数 (GI) ,软琼脂克隆形成试验检测细胞克隆形成率。结果 :10～ 36 0 μg/ml和 30～ 36 0 μg/ml 10 -HCPT分别作用QGY和HepG2细胞 4 8h后 ,均有显著性生长抑制作用 (P<0 .0 5 ) ,且呈量效依赖关系。 10 0 μg/ml10 -HCPT作用 2 4h、36、4 8和 72h对两种细胞均具有显著性抑制作用 (P <0 .0 5 )。实验组两种细胞克隆形成率较对照组均显著降低 (P <0 .0 5 )。结论 :10
Objective:To investigate the effects of 10-HCPT on the growth and proliferation of human hepatoma cell lines QGY and HepG2 in vitro.Methods:QGY and HepG2 were cultured in RPMI1640.They were treated by different concentrations of 10-HCPT for 48 hours,and by a dose of 100μg/ml of 10-HCPT for different hours.The cell growth indexes (GI) were detected by MTT colorimetric assay.The clone formation rates of QGY and HepG2 were observed by soft agar assay.Results:The GI of HepG2 and QGY cells treated by 10-HCPT with concentration 10～360μg μg/ml and 30～360μg μg/ml were significantly lower than that of control groups respectively ( P <0.05),and the inhibitive effect increased with concentrations ranging from 30 to 360μg/ml in a dose-dependent manner.The effects of 100μg/ml10-HCPT on the growth of QGY and HepG2 cells for 24 hours were significant as compared with control groups( P< 0.05).The inhibition in 36～72 hours was highly significant ( P <0.01),while not significant among themselves ( P >0.05).The clone formation rates of QGY and HepG2 of experimental groups were significantly lower than that of control groups respectively ( P <0.01).Conclution:10-HCPT could inhibit the growth and proliferation of human hepatoma cell lines QGY and HepG2 effectively in vitro.
出处
《重庆医科大学学报》
CAS
CSCD
2004年第3期305-307,共3页
Journal of Chongqing Medical University
