摘要
用PCR方法扩增了一株石油降解菌株G5的 16SrRNA基因全序列 ,并对其进行了克隆和测序 .对该序列在GenBank中的BLAST结果表明 ,所有与该序列高度同源的序列都是假单胞菌的 16SrRNA基因 .其中假单胞菌的代表菌株Pseudomonasaeruginosa,P .fluoroscens,P .putida ,P .syringae的 16SrRNA基因序列与G5的 16SrRNA基因序列同源性分别为 93.4 % ,98.4 % ,96 .3% ,97.5 % .对G5和其他 39株假单胞菌的 16SrRNA基因序列进行聚类分析 ,获得的系统发育树与RDP(RibosomalDatabaseProject)报道的系统发育树基本一致 ,其中菌株G5与 5株P .chlororaphis聚类在一起 .图 2参
The 16S rRNA gene of bacteria strain G5, a petroleum degrading strain, was amplified, cloned and sequenced. BLAST of the sequence in GenBank indicated that the species with close similarity to G5 were from genus Pseudomonas only. The similarity values of 16S rDNA sequence between strain G5 and typical species P. aeruginosa , P. fluoroscens , P. putida and P. syringae were 93.4%, 98.4%, 96.3% and 97.5%, respectively. The phylogenetic tree was produced from 16S rDNA of G5 and other 39 Pseudomonas species using 16S rDNA of E. coli as outgroup. Strain G5 was clustered with 5 strains of P. chlororaphis . Fig 2, Ref 7
出处
《应用与环境生物学报》
CAS
CSCD
2004年第3期363-365,共3页
Chinese Journal of Applied and Environmental Biology
