摘要
从水产养殖污泥中分离筛选了一株优良的亚硝酸盐降解菌AQ-3,其在1.0×107 cfu/mL时对50mg/L亚硝酸盐的去除率高达99.47%。通过细菌自动鉴定系统(API ID32GN系统)以及16SrDNA系统发育分析,菌株AQ-3被鉴定为鲍曼氏不动杆菌(Acinetobacter baumannii)(JF751054),其16SrDNA序列与基因库中不动杆菌属菌株的16SrDNA序列有99%的同源性,而且与鲍曼氏不动杆菌菌株14(FJ907197)的亲缘关系最近。此外,菌株AQ-3同时含有nirS基因、nirK基因和norB基因,这些基因对AQ-3高效降解亚硝酸盐具有重要的意义。
A potential nitrite degrading bacterium AQ-3 was isolated and screened from the aquaculture sediment.The nitrite removal rate of AQ-3 was detected as 99.47%.Strain AQ-3 was identified as Acinetobacter baumannii（JF751054） through API ID32GN bacterial identification system and 16S rDNA sequence analysis.Its 16S rDNA sequence had homology of 99% with those of Acinetobacter sp.submitted to GenBank,and showed the closest relative to Acinetobacter baumannii strain 14（FJ907197）.In addition,nirS gene、nirK gene and norB gene all existed in strain AQ-3.This study proved strain AQ-3 was a potential nitrite degrading bacterium,and enriched the microbial resources of nitrite degrading bacterium.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2012年第4期32-36,共5页
Environmental Pollution & Control
基金
国家"863计划"项目(No.2011AA10A216)
国家自然科学基金资助项目(No.31172430)
公益性行业(农业)科研专项(No.201203085)
现代农业产业技术体系建设专项(No.CARS-46)
关键词
亚硝酸盐
鲍曼氏不动杆菌
鉴定
系统发育分析
nitrite
Acinetobacter baumannii
identification
phylogenetic analysis
作者简介
王会聪，女，1985年生，硕士研究生，研究方向为渔用微生态制剂的研发与药物残留检测。
通讯作者。
