摘要
对pepc、ppdk和pepc +ppdk三种转基因水稻农艺性状观察表明 ,与原种Kitaake相比单株有效穗有不同程度的增多 ,单株产量相应提高 ,特别是pepc和ppdk基因聚合后 ,单株有效穗和单株产量分别比受体亲本Kitaake提高 2 9.1%和2 7.0 %。三种转基因材料作基因供体分别与受体光敏核不育系培矮 6 4S、2 30 4S和 2 30 6S杂交后 ,这些基因在新的遗传背景下不仅稳定遗传和高水平表达 ,而且表现增穗增产 ,特别当pepc和ppdk基因聚合时 ,与受体相比 ,F1的PEPC活性提高 5 .8～ 18.6倍 ,PPDK活性提高 0 .5～ 1.3倍 ,植株饱和光合速率提高 5 0 %左右。转育的转基因材料结实率有所降低 ,是值得进一步研究的问题。
Investigation of main agronomic traits for 3 types of transgenic rice with maize C 4 photosynthesis gene (i.e. pepc, ppdk, and pepc+ppdk gene) showed that the transgenic rice plant with the maize pepc and/or ppdk genes had more panicles and higher yield than untransgenic one. While pepc and ppdk genes were introduced into the same plant of Kitaake the panicle per plant increased by 29.1%, the grain weight per plant increased by 27.0% compared with untransgenic Kitaake plants. Using conventional crossing method, we have introduced maize pepc and ppdk genes into photo-sensitive genic male sterile lines Peiai64S, 2304S and 2306S respectively, and they are stably inherited in a Mendelian manner. Testing of the enzyme activities in these transgenic plants had showed a high level expression of the genes. With pepc and ppdk genes gathered together in the same plant, the activities of PEPC and PPDK were increased 5.8—18.6 folds and 0 5—1.3 folds respectively in F 1 plants, the saturated photosynthetic rates were about 50% higher than the receptor parents. The problem of lower seed setting should be studied further.
出处
《作物学报》
CAS
CSCD
北大核心
2004年第3期248-252,共5页
Acta Agronomica Sinica
基金
国家高技术研究发展计划 ( 863计划 )项目 ( 2 0 0 1AA2 11171
2 0 0 2AA2 0 70 0 1)
国家转基因植物研究与产业化专项 (J2 0 0 0 B 0 0 5 )
