摘要
用原种粳稻 Kitaake和转 PEPC基因水稻为材料 ,比较光合速率、叶绿素荧光参数、叶绿素含量等光合特性的差别。结果表明 ,转 PEPC基因水稻的饱和光强为 12 0 0μm ol/m2 .s,比原种高 2 0 0μmol/m2 .s,光饱和光合速率比原种高 5 0 %以上。在高光强 14 0 0μm ol/m2 .s(2 h)下 ,与原种相比 ,转 PEPC基因水稻的 PS 光化学效率 (Fv/Fm )下降较少、光化学猝灭系数 (q P)较恒定。在光氧化处理后 ,与原种相比 ,转 PEPC基因水稻的 Fv/Fm和光合速率的下降、叶绿素含量和组分的衰减较慢。上述结果表明 :转 PEPC基因水稻对高光强有较高的适应能力 。
The photosynthetic rate, parameters of chlorophyll fluorescence and chlorophyll content of PEPC transgenic rice were measured under photoinhibition conditions. The results showed that photosynthetic light saturation intensity of transgenic rice was 1200 μmol/m 2.s, which is 200 μmol/m 2.s higher than that of untransformed rice. The light saturation photosynthetic rate of transgenic rice was 50% more than that of untransformed rice. The photochemical efficiency of PSⅡ(Fv/Fm) decreased more slowly, and the photochemical quenching(qP) was not inhibited in transgenic rice as compared with untransformed rice. The content of chlorophyll and its components, the Fv/Fm and photosynthetic rate of transgenic rice also decreased more slowly than those of untransformed rice. According to these results: it could be seen that PEPC transgenic rice was more adaptable to high light intensity, and more tolerant to photoinhibition and photooxidation.
出处
《作物学报》
CAS
CSCD
北大核心
2002年第3期321-326,共6页
Acta Agronomica Sinica
基金
国家重点基础研究发展规划项目 (G19980 10 10 0 )
江苏省教委自然科学研究基金项目 (98KJB180 0 0 4)
