摘要
背景与目的:结直肠腺癌的发生是多因素作用的结果,survivin基因和PTEN蛋白均参与其中。虽然两者均参与细胞周期调控和细胞凋亡,但两者生物学效应却是相反的。它们在结直肠腺癌中所起的作用及相互关系尚不明确。本研究旨在探讨survivin与PTEN在结直肠腺癌发病中的作用及其关系。方法:对42例结直肠腺癌及20例相应的癌旁正常组织用逆转录PCR方法检测survivinmRNA的表达,免疫组织化学SP法检测PTEN蛋白表达。结果:结直肠腺癌组织survivin基因阳性率为54.8%,癌旁正常组织均无表达。Survivin的表达与结直肠腺癌分化程度、Dukes分期有关,而与性别、淋巴结转移情况无关。PTEN蛋白在结直肠腺癌中的阳性率(47.6%)低于癌旁正常组织(90.0%),两者差异有统计学意义(P<0.01)。杨冬,等.Survivin基因与PTEN蛋PTEN蛋白的表达与患者的性别无关,但与肿瘤分化程度、淋巴结转移有较密切关系,与Dukes分期可能有关。随着肿瘤恶性程度加重,survivin表达上调,PTEN表达下降。结论:Survivin基因和PTEN蛋白表达与结直肠腺癌临床病理特征和生物学行为有密切关系,联合检测survivin和PTEN,对诊断大肠腺癌、判断其恶性程度及预后均有一定参考价值。
BACKGROUND & OBJECTIVE: The occurrence of colorectal adenocarcinom a is resulted from multiple factors and both survivin gene and PTEN protein take part in these courses. They all regulate cell cycle and apoptosis, but their bi ological effects are adverse. It is not completely elucidated about their functi on and relationship in colorectal adenocarcinoma. This study was designed to inv estigate the role of survivin gene and PTEN protein in the pathogenesis of color ectal adenocarcinoma and their correlation. METHODS: To determine the survivin m RNA in 42 colorectal adenocarcinoma and 20 adjacent normal colorectal tissue sam ples, we used reverse transcription polymerase chain reaction (RT-PCR) to deter mine the expression of survivin mRNA, and immunohistochemical evaluation was use d to determine the expression of PTEN protein. RESULTS: The positive expression rate of survivin was 54.8% in colorectal adenocarcinoma, while no expression w as detected in adjacent normal tissue. The expression of survivin gene was signi ficantly correlated with histological differentiation and Dukes stage, but not w ith gender or lymph node metastasis. The positive rate of PTEN protein in colore ctal carcinoma was lower (47.6%) than that in adjacent normal tissue (90.0%). The difference between them was significant (P< 0.01). There was no relationsh ip between PTEN protein and gender, while the expression of PTEN was positively correlated with histological differentiation and lymph node metastasis, and poss ibly correlated with Dukes stage. With the progress of tumor in malignancy, nega tive correlation was observed between survivin and PTEN expression in colorectal adenocarcinoma. CONCLUSION: Survivin gene and PTEN protein are significantly co rrelated with the clinicopathological characteristics and biologic behaviors in colorectal adenocarcinoma. Detection of survivin together with PTEN is valuable for diagnosing colorectal adenocarcinoma, and evaluating malignancy extent and p rognosis.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2004年第3期306-309,共4页
Chinese Journal of Cancer
