摘要
目的 :观察大肠腺瘤和腺癌组织中 PTEN、缺氧诱导因子 - 1α(HIF- 1α)表达情况 ,探讨两者在大肠腺瘤一腺癌序列发生发展中的作用及相互关系。方法 :采用原位杂交技术检测 18例大肠腺瘤和 6 2例大肠腺癌组织中 PTEN- m RNA、HIF-1αm RNA,用免疫组织化学 S- P方法检测 VEGF蛋白的表达。比较三者与大肠癌 Dukes分期、淋巴结转移、肝转移及组织学分级等病理特征的关系。结果 :18例大肠腺瘤 PTEN- m RNA、HIF- 1αm RNA、VEGF蛋白阳性表达率分别为 77.8% (14 / 18) ,38.9% (7/ 18) ,33.3% (6 / 18) ,大肠腺癌分别为 5 1.6 % (32 / 6 2 ) ,6 7.7% (42 / 6 2 ) ,5 9.7% (37/ 6 2 )。腺瘤组织 HIF- 1αm RNA、VEGF阳性表达率均低于腺癌细织 (P <0 .0 5 ) ,腺瘤组织 PTEN- m RNA阳性表达率显著高于腺癌组织 (P <0 .0 5 )。大肠腺癌细织中 PTEN- m RNA吸光值随 Dukes分期增高而逐渐降低 ,A期 (0 .1782± 0 .0 2 71) ,B期 (0 .15 38± 0 .0 397)分别与 C+D期(0 .14 70± 0 .0 5 2 4 )比较差异均具有显著性 (P <0 .0 5 )。 HIF- 1αm RNA、VEGF蛋白吸光值随 Dukes分期增高而逐渐升高 ,A期 ,HIF- 1α(0 .10 2 9± 0 .0 4 5 ) ,VEGF(0 .12 0 7± 0 .0 4 36 )分别与 B期 (0 .16 5 6± 0 .0 32 9、0 .15 72± 0 .0 5
Objective:To investigate the expressions and relationship of PTEN,hypoxia-inducible factor-1 alpha(HIF-1α ) in colorectal carcinogenesis.Methods:71 cases of formalin-fixed, paraffin-embedded colorectal neoplasms specimens (18 cases of adenomas, 62 cases of adenocarcinomas) were used. PTEN mRNA, HIF-1 α mRNA were detected by in situ hybridization. VEGF proteins were identified by citrate-microware SP immunohistochemical method.Results:The expressions of PTENmRNA, HIF-1 α mRNA, VEGF protein were detected in 14, 7,6 cases of 18 colorectal adenomas respectively. Significant difference was observed in positive rates of PTEN, HIF-1 a and VEGF expressions between colorectal adenomas and adenocarcinoma(P<0. 05).The levels of PTEN decreased as the pathologic stage increased. Reversely HIF-1 a and VEGF expressions increased with the specimen's dukes stage as follows: stage A(0. 1029±0. 045; 0. 1207±0. 0436), stage B(0. 1656±0. 0329;0. 1572±0. 0514), stage C+D (0. 2335±0. 0748;0. 2219±0. 0803). Significant differences of PTEN mRNA ,HIF-1α mRNA and VEGF were found between dukes stage A(0. 1782± 0.0271) and stage B(0.1538±0. 0397).The levels of PTEN in stage C+D were statically higher than stage A and B(P<0.05).HIF-1α and VEGF expressions correlated significantly with lymph node metastasis, liver metastasis and Duke' s stage. In colorectal carcinoma reduced levels of PTEN were statically significantly associated with increased expressions of HIF-1α mRNA (r s=-0.36,P<0.05) and VEGF protein (r s=-0.68, P<0.05) respectively. The levels of HIF-1 were correlated with VEGF expression (r s=0.71,P<0.01).Conclusion:The absence or low expression of PTEN and the increased levels of HIF-1α and VEGF may play an important role in carcinogenesis and progression of colorectal carci noma.
出处
《大肠肛门病外科杂志》
2004年第4期241-245,共5页
Journal of Coloproctological Surgery
基金
湖北省科技攻关项目 (编号 :2 0 0 3 AA3 0 1C0 3)
