摘要
目的研究中药复方喉咽清对甲型H1N1流感病毒感染小鼠肺炎模型的治疗作用。方法采用ICR小鼠随机分为正常组,模型组,喉咽清高、中、低剂量组(15.60,7.80,3.90 g·kg-1),阳性对照药磷酸奥司他韦胶囊组(0.04 g·kg-1),采用滴鼻感染甲型H1N1流感病毒FM1株建立小鼠流感病毒肺炎模型,给药5 d,感染第6日测定小鼠体重、肺指数值以及14 d内小鼠存活时间、死亡率,感染第3、6、9日采用Real time PCR方法检测小鼠肺组织中病毒载量表达。结果与模型组比较,喉咽清高剂量组小鼠肺指数值、死亡率明显降低,喉咽清高、中、低剂量组体重明显增加,喉咽清高、中剂量组存活时间明显延长,感染第6、9日喉咽清高剂量组肺组织病毒载量明显降低。结论喉咽清对甲型流感病毒性肺炎具有治疗作用,其可能是通过降低小鼠肺组织中流感病毒载量发挥作用,该作用值得进一步研究。
Objective To determine the therapeutic effect of Houyanqing on pneumonia mice model infected with influenza A virus(H1 N1).Methods The mice were randomly divided into a normal group,a model group,Houyanqing high,medium and low dose groups(15.60,7.80,and 3.90 g·kg-1),and oseltamivir phosphate capsule group(0.04 g·kg-1).H1 N1 virus FM1 strain was used to establish the model of influenza virus pneumonia by nasal drip.The drug was orally administered for 5 days.The weight,lung index,survival time and mortality rate were measured.The viral load in the lung tissues of mice detected by Real time PCR was observed on day 3,6,and 9.Results The body weight of high,medium,and low dose Houyanqing groups was much higher than that of the model group.The lung index and mice mortality of the high dose Houyanqing group were obviously lower than those of the model group.The survival time of mice in Houyanqing high and medium dose group was longer than that in the model group.The viral load of Houyanqing high dose group was much lower than that of the model group on day 6 and 9.Conclusion Houyanqing has therapeutic effect on H1 N1 by downregulating the viral load in the lung tissue of mice.
作者
孙建辉
唐纯玉
霍海如
李小芹
卫军营
唐代凤
李洪梅
杨洪军
SUN Jian-hui;TANG Chun-yu;HUO Hai-ru;LI Xiao-qin;WEI Jun-ying;TANG Dai-feng;LI Hongmei;YANG Hong-jun(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing100700;Hunan Timesun Pharmaceutical Co.,Ltd.Yongzhou Hunan425006;Hunan EngineeringTechnology Research Center for Anti-Infection Traditional Chinese Medicine,Yongzhou Hunan425006)
出处
《中南药学》
CAS
2019年第8期1229-1233,共5页
Central South Pharmacy
基金
2016年湖南省人才工作专项资金-时代阳光现代中药科技创新创业团队支持计划任务(编号:湘科人[2016]2号)
2017年度科技特派员创新创业项目-新增国家医保目录品种喉咽清颗粒标准体系提升研究(编号:湘财教指[2017]131号)
关键词
喉咽清
肺指数
存活时间
死亡率
病毒载量
Houyanqing
lung index
survival time
mortality rate
viral load
作者简介
孙建辉,男,主管技师,主要从事中药药理及新药研发,E-mail:sjh121858@sina.com;通讯作者:李洪梅,女,博士,副研究员,主要从事中药药理研究及新药开发,E-mail:lihm2006@sina.cn
