摘要
目的:探讨当归提取物对^(60)Coγ电离辐射损伤小鼠的脾脏组织蛋白质差异表达的影响及作用机制。方法:通过^(60)Coγ射线照射昆明种小鼠构建电离辐射损伤模型。运用iTRAQ联合LC-MS/MS技术对小鼠脾脏组织进行蛋白质组学检测,采用DAVID 6.8对差异蛋白进行Gene Ontology(GO)分析和KEGG Pathway显著富集性分析。结果:收集到240个差异蛋白参与的10条KEGG信号通路(P<0.05),其中细胞色素B-245、髓过氧化物酶、主要组织相容性复合体、细胞表面糖蛋白TROP2等四个差异蛋白在吞噬体和抗原处理与呈递两条通路富集比较明显。结论:在小鼠脾脏组织中当归提取物抗电离辐射损伤的差异蛋白主要富集于吞噬体和抗原处理与呈递通路,当归提取物抗电离辐射损伤的机制可能与吞噬体和抗原处理与呈递参与的免疫调节相关。
OBJECTIVE To investigate the effect of angelica extracts on the differential expression of protein and possible mechanisms in spleen tissue of mice damaged 60Coγionizing radiation.METHODS The ionizing radiation injury model was constructed by irradiating Kunming mice with 60Coγray.Proteomics analysis of mouse spleen tissues was performed by iTRAQ combined with LC-MS/MS,and differential proteins were analyzed for significant enrichment by Gene Ontology(GO)and KEGG pathway using DAVID 6.8.RESULTS Ten KEGG pathways involved in 240 differentially expressed proteins were identified(P<0.05),among which cytochrome B-245,myeloperoxidase,major histocompatibility complex and cell surface glycoprotein TROP2 were significantly enriched in phagosome and antigen processing and presentation pathways.CONCLUSION The differentially expressed proteins of angelica extracts against ionizing radiation injury in mouse spleen tissues are mainly enriched in phagosome and antigen processing and presentation pathways,which may be related to the immune regulation involved in phagosomes and antigen processing and presentation.
作者
易辉燕
李敏
罗芳芳
吴莎莎
董军
王继生
YI Hui-yan;LI Min;LUO Fang-fang;WU Sha-sha;DONG Jun;WANG Ji-sheng(Schoolof pharmacy of Southwest Medical University,Sichuan Luzhou 646000,China;The Third People’s Hospital of Mianyang(Sichuan Mental Health Center),Sichuan Mianyang 621000,China)
出处
《中国医院药学杂志》
CAS
北大核心
2019年第9期899-904,共6页
Chinese Journal of Hospital Pharmacy
基金
四川省科技厅资助项目(编号:2014JY0058)
关键词
电离辐射
当归提取物
脾脏
蛋白质组学
吞噬体
抗原提取与呈递
ionizing radiation
angelica extracts
spleen
proteomics
phagosomes
antigen extraction and presentation
作者简介
易辉燕,女,硕士生,主管药师,研究方向:药剂学与临床药学,电话:0816-2270285,E-mail:903827475@qq.com;通讯作者:王继生,男,博士,主任药师,硕士生导师,研究方向:神经精神药理及药物蛋白质组,电话:0816-2270102,E-mail:24475434@qq.com
