摘要
Objective: To investigate the relationship between the polymorphism of myeloperoxidase (MPO) gene and coronary artery injury in children with Kawasaki disease (KD) and the therapeutic effect of human immunoglobulin (IVIG) combined with heparin. Methods: A total of 78 KD children and 65 normal children treated in our hospital from April 2016 to February 2018 were selected as the s study ubjects. They were divided into coronary artery injury group (36 cases) and non-coronary artery injury group (42 cases) according to whether or not coronary artery injury occurred. Polymerase chain-endonuclease fragment (PCR-RFLP) was used to determine the distribution of single nucleotide polymorphism (SNP) locus polymorphism of MPO gene. Clinical data of children were collected to compare the distribution of genotype frequency and allele frequency among different groups. All KD children were treated with IVIG combined with low molecular weight heparin calcium subcutaneously. The correlation between MPO gene polymorphism and therapeutic effect was analyzed. Results: The frequencies of three genotypes (AA, AG and GG) in KD group were 1.28%, 20.51% and 78.21% respectively, while those in control group were 9.23%, 63.08% and 23.08% respectively. There was a significant difference between the two groups;the allele frequency of G in KD group was 88.46%, significantly higher than 76.92% in control group. The frequency distribution of each genotype in coronary artery injury group was significantly different from that in non-coronary artery injury group. The frequency of G allele in coronary artery injury group was significantly higher than that in non-coronary artery injury group. The total effective rate of KD group was 75.64%. The total effective rate of AG+AA genotype and GG genotype was 94.12% and 70.49%, respectively. The difference was statistically significant. There was no significant difference in coronary artery diameter between different genotypes before treatment, and after treatment, the GA+AA genotype's coronary artery diameter was significantly reduced compared with GG genotype's;the total fever time, the time of congestion and edema subsidence, the time of lymphadenopathy subsidence in GA+AA genotype were significantly shorter and those in the GG genotype. Conclusion: The SNP polymorphism of MPO gene may be related to the occurrence of coronary artery injury in children with KD, and G allele may be a risk factor. In addition, the polymorphism of MPO gene is associated with the efficacy of IVIG combined with heparin in the treatment of KD, which may be an effective predictor of the efficacy of IVIG combined with heparin.
作者简介
Corresponding author:Zong-Xiu Zhao,Chengdu Women's and Children's Central Hospital,Chendu 610031.Email:2466181092@.qq.com
