摘要
成功构建了含冷诱导转录因子CBF3(CRT binding factor)的植物表达载体,通过农杆菌介导法转入三月茄,采用PCR检测DNA和卡那霉素涂抹叶片法进行田间抗性鉴定,证实获得含抗寒基因CBF3的茄子转基因植株2株,建立了抗寒的茄子再生与转化技术体系。
We successfully constructed cold-induced transcription factor CBF3(CRT binding factor),which was transferred to eggplant‘Sanyueqie’(Solanum melongena L.)by agrobacteriummediated transformation. And we obtained 2 transgenic eggplant plants containing cold-tolerant gene CBF3,through Polymerase Chain Reaction(PCR)detection genomic DNA,and kanamycin solution daubing on the leaves of generation plants in the field confirmed that the transgenic plants of eggplant were positive.Thus,we successfully established regeneration and transformation systems in eggplant.
出处
《中国蔬菜》
北大核心
2013年第05X期36-43,共8页
China Vegetables
基金
贵州省优秀青年科技人才培养对象专项资金(黔科合人字〔2009〕03号)
贵州省优秀科技教育人才省长专项资金(黔省专合字〔2009〕61号)
贵州省科技重大专项(黔科合重大专项字〔2011〕6001)
贵州省现代农业产业技术体系建设项目(GZCYTX2011-0101)
关键词
茄子
冷诱导转录因子CBF3
遗传转化
Eggplant
Cold-induced transcription factor CBF3
Genetic transformation
