摘要
以抗病品种G087和感病品种G025为试材,研究了大蒜紫斑病菌粗毒素对大蒜幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性和丙二醛(MDA)含量及叶片解剖结构的影响。结果表明:在大蒜紫斑病菌粗毒素处理后120h内,抗病品种和感病品种叶片SOD活性呈现一致的变化趋势,均在96h达到峰值,但抗病品种SOD活性始终高于感病品种。随着大蒜紫斑病菌粗毒素处理时间的延长,感病品种叶片POD和PAL活性均呈先持续升高后稍下降的趋势,均有1个峰值;而抗病品种叶片POD和PAL活性均出现2个峰值,POD活性的2个峰值分别出现在48h和96h,PAL活性的2个峰值分别出现48h和120h;抗病品种酶活性的最大峰值高于感病品种。在大蒜紫斑病菌粗毒素处理后的大部分时间内,抗病品种叶片MDA含量低于感病品种。抗病品种叶肉组织结构比感病品种紧密;经大蒜紫斑病菌粗毒素处理后,两个品种叶片栅栏组织及海绵组织的密度都比处理前增加,抗病品种叶肉组织结构比感病品种更加紧密。
The effects of Alternaria porri(Elliott)Cif.crude toxin on the activities of SOD,POD,PAL and contents of MDA and leaf anatomical structure were studied,taking the resistant cultivar G087 and susceptible cultivar G025 of garlic(Allium sativum L.)as experiment materials.The results showed that the SOD activities of both cultivars appeared the same dynamic changing trend in 120 hours after the pathogen crude toxin treatment and reached the peak at 96 hours.But the SOD activities in resistant cultivar were higher than that in susceptible cultivar.The POD and PAL activities in the leaves of susceptible cultivar showed a trend of sustaining increase first then a slight decrease and had a peak value.However,the activities of POD and PAL in the leaves of resistant cultivar had 2 peak values,which presented at 48 hours and 96 hours for POD and 48 hours and 120 hours for PAL,respectively after pathogen crude toxin treatment.The activities of both enzymes in resistant cultivar were higher than that in the susceptible cultivar at the peak.The MDA content in the leaves of resistant cultivar was lower than that in the susceptible cultivar at the most time after pathogen crude toxin treatment.The mesophyll tissue in the leave of resistant cultivar was more compact than that in susceptible cultivar.The leaf palisade tissue and spongy tissue became more compact in both cultivars after pathogen crude toxin treatment,but the mesophyll tissue structure in the resistant cultivar became more compacter than that in susceptible cultivar.
出处
《中国蔬菜》
北大核心
2012年第08X期68-74,共7页
China Vegetables
基金
国家公益性行业(农业)科研专项(200903018-7)
西北农林科技大学唐仲英育种基金项目(A212020911)
关键词
紫斑病菌
粗毒素
大蒜
保护酶
叶片解剖结构
Alternaria porri(Elliott)Cif.
Crude toxin
Garlic
Protective enzymes
Leaf anatomical structure
