摘要
目的 :建立一种制备方法简便、具有高存活率及成模率的肝硬化、门脉高压伴腹水的大鼠动物模型。方法 :80只重量为 2 0 0～ 2 5 0 g的雄性SD大鼠 ,随机选取 10只为正常对照组 ,其余 70只为肝硬化腹水造模组。造模组大鼠的处理 ,苯巴比妥溶液 (35mg % )作为饮用水诱导 1周。第 2周始每周给予 4 0 %CCl4-橄榄油溶液皮下注射 2次 ,每次剂量为 0 .2ml / 10 0g ,首剂加倍 ,第 9周始改为每 2周 3次直至第 14周。结果 :第 14周 ,肝硬化腹水造模组 70只大鼠存活 37只 ,其中 36只存在腹水。肝硬化腹水造模组的大鼠肝脾明显肿大 ;肝表面凹凸不平 ,可见大小结节 ;组织学检查显示肝硬化、假小叶的形成 ;门静脉压力较正常对照组明显升高 (17.6 7± 3.4 7vs 8.6 7± 1.0 3cmH2 O ,P <0 .0 1) ;血清总胆红素、谷丙转氨酶及谷草转氨酶较正常对照组明显升高 (P <0 .0 5 ) ,而血清白蛋白较之正常对照组明显降低 (P <0 .0 1)。结论 :本实验经对CCl4加苯巴比妥联合造模法加以改良 ,获得了一种肝硬化门脉高压伴腹水的大鼠模型 。
Objective: To establish a decompensated cirrhotic rat model complicated with ascites which is convenient and reliable with high survival rate. Methods: 70 adult male SD rats whose weight was over 200g were fed with phenobarbital solution (35mg%) daily during the first week (induction phase). They were administrated with 40% CCl 4-olive oil mixture by subcutaneous injection (0.2ml /100g) twice a week for 8 weeks.Then the injections decreased to three times every two weeks for next 6 weeks.After 14 weeks,ascites was determinated by the measurement of abdomen circumference and abdominal paracentesis. Results: After 14 weeks,37 rats survived in which 36 rats had ascites.The pathological study showed the formation of liver cirrhosis.Splennomegaly was also present. Portal vein pressure increased. Rats in normal control group showed no cirrhosis.The levels of serum total bilirubin(TB)?alanine aminotransferase(ALT) and oxalacetic transaminase (AST) were higher in cirrhotic rats than normal rats(P<0.01) while the level of serum albumin were lower in cirrhotic rats(P<0.05). Conclusions: A decompensated cirrhotic rat model complicated with ascites was successfully established by improving the previous method of CCl 4 plus phenobarbital.
出处
《中国临床医学》
2003年第2期154-156,共3页
Chinese Journal of Clinical Medicine
