摘要
210 .seq是一个在大鼠心肌缺血预适应中表达上调的表达序列标签 (expressedsequencetag ,EST) ,用GENSCAN预测、Blast(Basiclocalalignmentsearchtool)比对、序列拼接、多序列比对等生物信息学方法克隆了该EST代表的大鼠MIP3基因的cDNA序列。大鼠MIP3基因的开放阅读框为 1332bp ,起始密码子前同一相位存在一个终止密码子 ,起始密码子邻近的序列符合Kozak规则 ;推测编码 4 4 3个氨基酸 ,与小鼠和人的MIP3基因or tholog的同源性很高 ,但是与数据库中其它蛋白质的同源性不高。TMpred分析显示该多肽有一个跨膜区域 ,氨基端位于膜内。Motifscan分析仅发现一个脯氨酸富集区域。
seq was an expressed sequence tag (EST) which expressed more in rat myocardium with ischemic preconditioning treatment than that with sham operation. We cloned the cDNA sequence of rat myocardium ischemia preconditioning 3 ( MIP3 ) gene based on 210.seq using GENSCAN, alignment, assembling and other programs. The open reading frame of 210.seq was 1332 bp. A stop codon was located in the same frame upstream from the start codon. The flanking sequences of start codon accorded with Kozak rule. The predicted polypeptide was 443 amino acids, which showed high homology with MIP3 ortholog of mouse or human, but little homology with other proteins in the database. TMpred analysis revealed that the polypeptide had one transmembrane region with N terminus inside. Motifscan analysis showed that it had only one proline rich region, and MIP3 gene belonged to a novel gene with unknown function.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第5期441-444,共4页
Bulletin of Hunan Medical University
基金
国家 973重点项目 (G2 0 0 0 0 5 690 8)
国家自然科学基金 ( 3 0 170 3 73 )
