摘要
目的筛选新的菊酯农药降解酶基因并了解其特性,为解决食品和环境中的菊酯农药残留问题创造条件。方法通过构建基因文库的方式筛选获得新的菊酯农药降解酶(EstP)基因,并对其进行一系列生物信息学分析。结果获得了新的菊酯农药降解酶(EstP)基因。EstP属于水解酶类,由638个氨基酸编码,预测分子量为73.4 kDa,pI值为8.34,与其他蛋白相似性极低,不属于任何已知的蛋白超家族,仅与羧肽酶Taq(M32)的保守域具有一定相似性,推测Lys137,Glu116,Glu148为其必需氨基酸。结论新的菊酯农药降解基因得到克隆,生物信息学分析为其定向进化提供了理论指导,为高效基因工程菌的构建打下了基础。
Objective To obtain pyrethroid-hydrolyzing esterase （EstP） gene and analyze the characteristics of it. Methods Bioinformatics analyzing tools were used to screen a EstP gene from Klebsiella sp. Strain ZD112 DNA library and predict the character and function of the protein. Results A novel EstP with higher activity was found. EstP was a protein of 638 amino acid residues, a molecular mass of 73.4 kDa, and pI 8.34. It showed no similarities with known proteins, and did not belong to any protein superfamily. It is expected to be related to carboxypeptidase Taq （M32） metallopeptidase, and revealed that Lys136, Glull4, Glu147 were important for the esterase activity. Conclusion A new EstP gene was obtained, which might lay a basis for the further study on the structure analysis and gene clone of it.
出处
《广东药学院学报》
CAS
2008年第3期277-281,共5页
Academic Journal of Guangdong College of Pharmacy
基金
广东省自然科学基金(031618)
广东省科技计划重点资助项目(C32402)
关键词
菊酯农药降解酶
基因克隆
菊酯农药
生物信息学分析
pyrethroid-hydrolyzing esterase
molecular cloning
pyrethroid insecticides
bioinformatic analysis
作者简介
王卓娅（1981-），女，硕士，助教；
通信作者：李荷，女，博士，副教授，主要从事微生物水解酶及分子生物学研究，E-mail：lihe32@163．com.
