摘要
为实现草鱼内脏蛋白质的高值化利用,本研究在单因素试验基础上应用响应面试验优化了木瓜蛋白酶水解脱脂草鱼内脏蛋白质的条件,响应面法分析得出4个影响因素的最佳组合为加酶量33 015 U/g、酶解温度62.35℃、酶解时间5.37 h、酶解p H 7.9,此时水解度为44.48%,肽得率为19.68%,水解液鲜味较明显。
The hydrolysis conditions of defatted visceral waste proteins of grass carp with papain were optimized by the combined use of single factor method and response surface methodology. An enzyme dosage of 33 015 U/g, a hydrolysis temperature of 62.35 ℃, a hydrolysis time of 5.37 h and initial p H 7.9 were found to be optimal for the hydrolysis of visceral proteins of grass carp. Under these conditions, the degree of hydrolysis(DH) was 44.48% with a peptide yield of 19.68%, and the hydrolysate had a medium umami taste.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第10期43-48,共6页
Food Science
基金
北京市自然科学基金项目(6132004)
北京市科技新星计划项目(Z131102000413005)
关键词
草鱼内脏
蛋白质
水解
响应面试验
优化
grass carp viscera
protein
hydrolysis
response surface methodology
optimization
