摘要
目的:研究荷叶提取物(乙醇提取物、生物碱、总黄酮和小分子水溶物)对小鼠的镇静催眠作用,筛选其活性组分。方法:利用空场实验、戊巴比妥钠阈下催眠剂量实验和延长戊巴比妥钠睡眠时间实验,给予小鼠灌胃荷叶50%乙醇提取物(150、450 mg/kg)和不同分离组分(30、90 mg/kg)后,观察其对小鼠的镇静催眠作用。结果:高剂量荷叶乙醇提取物和低、高剂量荷叶生物碱均能显著抑制小鼠的自主活动,增加阈下剂量戊巴比妥钠引起的小鼠入睡率,缩短戊巴比妥钠阈上剂量的小鼠入睡潜伏期,延长睡眠时间。结论:荷叶乙醇提取物具有显著的镇静催眠作用,生物碱是其主要活性成分。
Objective:The present study aimed to determine the sedative and hypnotic effects of 50% ethanol extract and its different fractions prepared from Nelumbo nucifera leaves.Methods:Locomotion activity and pentobarbital-induced sleeping tests were used to evaluate the sedative and hypnotic activities of 50% ethanol extract(150 and 450 mg/kg) and different fractions(30 and 90 mg/kg) through gavage administration.Results:Ethanol extract(450 mg/kg) and alkaloid fraction(30 and 90 mg/kg) signifi cantly inhibited the locomotion activity of mice,increased the percentage of sleeping and decreased the sleep latency and prolonged the sleeping time of mice.Conclusion:Nelumbo nucifera leaves possess sedative and hypnotic effects and its alkaloids are the bioactive components for these activities.
出处
《食品科学》
CAS
CSCD
北大核心
2015年第5期168-171,共4页
Food Science
基金
科技部对欧盟科技合作专项(1108)
科技部国际科技合作项目(2011DFA32730)
关键词
荷叶
镇静
催眠
生物碱
Nelumbo nucifera leaves
sedative
hypnotic
alkaloids
