摘要
为获得可用于生产的高效降解猪血的菌株,在以猪血为主要培养物的平板上对生猪屠宰场下水道土壤中的微生物进行分离,根据微生物在酪素固态培养基上透明圈的大小,初筛得到10株产蛋白酶的菌株。发酵后测定酶活力,筛选出1株产酶能力较高的菌株B12。根据形态学分析、生理生化实验,以及16S rRNA序列比对,鉴定为Bacillus cereus,构建了关于B12的系统发育树。菌株B12在以葡萄糖为碳源,猪血为氮源,培养基初始p H 7.0,培养温度33℃的条件下振荡培养24 h,所产蛋白酶活力最高可达185.48 U/m L。
Porcine is a valuable protein resourse,but development of the porcine resource is limited deeply because the porcine protein molecule is too big to be absorbed,and the porcine seriously pollute the environment due to being discharged at random. Through enrichment culture with porcine protein,primary screen with casein plate,and secondary screen with fermented porcine protein,a microbial strain named B12 producing high activity protease was obtained from the slaughter house and its surrounding soil. Based on morphological observation,typical physio-biochemical tests and 16 S r DNA phylogenetic analysis,this strain was preliminarily identified as a species belonging to the Bacillus cereus. After being inoculated on the plate with glucose as carbon resource and with porcine as carbon resource,cultured under 33 ℃ and p H 7. 0 for 24 h,specific activity of protease produced by this strain could reach185. 48 U / mg. The strain B12 might be used in the industry of degrading the porcine.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第3期58-63,共6页
Food and Fermentation Industries
基金
江西省教育厅青年科学基金项目(GJJ11075)资助
关键词
猪血蛋白
产蛋白酶菌株
筛选
鉴定
产酶条件
porcine protein
protease-producing strain
screening
identification
fermentation condition
