摘要
目的建立动物血浆中克伦特罗、莱克多巴胺、沙丁胺醇的超高效液相色谱-串联质谱检测方法。方法采用乙酸铵缓冲液酶解提取血浆中的目标化合物,提取液经混合阳离子交换柱净化,采用超高效液相色谱-串联质谱(ultra performance liquid chromatography–tandem mass spectrometry)多反应监测模式检测,内标法定量。结果克伦特罗、莱克多巴胺、沙丁胺醇回收良好,线性相关系数均大于0.99,测定低限均为0.5μg/kg。结论该方法适用于动物血浆中克伦特罗、莱克多巴胺、沙丁胺醇残留的检测。
ABSTRACT:Objective A method based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) has been developed for the determination of beta-agonists residues including clenbuterol, ractopamine, and salbutamol in animal plasma.Method The analytes were extracted with NH4AC solution from samples and hydrolysised with beta-glucuronidase overnight at 37℃. The extract was filtered and the filtrate was cleaned by Oasis MCX solid phase extraction cartridge. Quantification of the 3 analytes was achieved by UPLC-MS/MS with multiple reaction monitoring (MRM) using internal standard method.ResultsThe method had a goodrecovery for the clenbuterol, ractopamine, and salbutamol, and the correlation coefficient was more than 0.99 with the limit of quantitation (LOQ) 0.5μg/kg.Conclusion The method is suitable for the determination of clenbuterol, ractopamine, and salbutamol residues in animal plasma.
出处
《食品安全质量检测学报》
CAS
2015年第12期5023-5028,共6页
Journal of Food Safety and Quality
基金
检验检疫行业标准(2013B324)~~
关键词
血浆
克伦特罗
莱克多巴胺
沙丁胺醇
超高效液相色谱-串联质谱法
plasma
clenbuterol
ractopamine
salbutamol
ultra performance liquid chromatography –tandem mass spectrometry
