摘要
目的 :建立新生大鼠缺氧缺血性脑损伤 (HIBD)模型 ,并讨论模型制作中的有关问题。方法 :健康 7日龄SD新生大鼠随机分为空白对照组 (n =18) ,假手术组 (n =8)和HIBD模型组 (n =19) ,采用经典Rice法制作新生大鼠HIBD模型 ,于 14日龄处死 ,观察HIBD后大鼠体重增长情况 ,左、右脑重比值变化 ,行为能力表现 ,以及脑组织病理形态学改变。结果 :①HIBD模型组体重增长明显慢于空白对照组和假手术组 (P <0 .0 1)。②HIBD组制成模型后全部发生行为异常改变 ,6 3 .16 %翻身不能 ,89.47%自发或夹尾左旋 ,抽搐占 47.37%。空白对照组和假手术组未见行为异常表现。③HIBD模型组左 /右脑重比值明显低于空白对照组和假手术组 (P <0 .0 1)。④肉眼观察HIBD组左脑萎缩者占 84.2 1%,出现软化灶占 31.5 8%,液化灶 5 2 .6 3 %,形成空洞脑者占 15 .79%;HE染色可见左侧大脑半球神经元损伤及神经胶质细胞增生 ;空白对照组和假手术组左、右脑无明显异常。结论 :本方法制作新生大鼠HIBD模型成功 ,该模型价格相对低廉、成功率高、重复性好、易于制作 ,值得进一步推广。模型制作中环境温度应保持在 36～ 37℃。
Objective To duplicate a model of hypoxic-ischemic brain damage (HIBD) in the neonatal rat to create unilateral cerebral damage.Methods Healthy 7-day-old Sprague-Dawley (SD) rats were used as research subjects and randomly divided into 3 groups: the control group (n = 18), the sham operated group (n = 8), and the HIBD group (n = 19). The HIBD model was produced according to the traditional Rice model and the brains were collected when the rats were 14 days old. The weight, left/right brain weight ratio, behavioral ability,and neuropathologic changes were studied in each group.Results ①The weight in the HIBD group increased obviously slowly and was lower than that in the control group and sham operated group (P <0.01). ②The abnormity of behavioral ability was found in the HIBD group, including turnover unability (63.16%), spontaneously turn left round (89.47%), convulsion (47.37%), and so on. ③The left/right brain weight ratio was significantly lower in the HIBD group than in the other two groups (P <0.01). ④The pathologic features in the HIBD group were as follows: atrophy of left brain (84.21%), softening of left brain (31.58%), liquefaction of left brain (52.63%), and cavum brain (15.79%). HE staining showed the neuron damage and the proliferation of neuroglial cells in the HIBD group. There was no obvious abnormity found in the control group and the sham operated group. Conclusion The model of HIBD is successful and worthy of spreading,with its cheap price, easy duplication, and higher successful rate. The reasonable ambient temperature should be kept in 36～37℃.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第2期133-136,共4页
Bulletin of Hunan Medical University
