摘要
目的探讨早期丰富环境干预对未成熟大鼠脑室周围白质软化(PVL)损伤后神经行为及生长相关蛋白(GAP-43)表达的影响。方法选择2日龄SD大鼠制作PVL模型,随机分为干预组、非干预组、假手术组、对照组,各40只。假手术组仅分离左侧颈总动脉,不予结扎和缺氧,非干预组和假手术组均饲养于标准环境中,不进行丰富环境干预。干预组于术后第4天行触摸和丰富环境干预,总干预时间为28d。干预结束后行神经行为学检测,同时分别于术后第4、11、18、25、32天取各组大鼠海马脑组织,采用RT-PCR技术检测GAP-43 mRNA表达。结果干预组大鼠于生后5周龄感觉运动功能及学习记忆能力较非干预组明显改善[(悬吊试验:(4.5±1.5)minvs(3.1±0.9)min;斜坡试验:(2.3±0.7)svs(3.9±1.1)s;逃避潜伏期:(4.86±2.94)svs(15.0±11.23)s;空间探索能力:(62.89±8.69)%vs(43.99±8.29)%Pa<0.05]。干预组海马GAP-43 mRNA表达于术后第11、18、25、32天的光密度比值较非干预组明显增高[(3.21±0.45)vs(2.63±0.32);(2.74±0.34)vs(2.47±0.25);(2.14±0.29)vs(1.75±0.19);(1.96±0.25)vs(1.63±0.15)Pa<0.01]。结论早期适度丰富环境干预可增强未成熟大鼠PVL脑功能的恢复,GAP-43增多可能是其脑功能恢复机制之一。
Objective To explore the protective effects of the early enriched environment stimulation in the periventricular leucomalacia （PVL） of the premature rats and the expression of growth - associated protein - 43 （ GAP - 43 ）. Methods Two days old postnatal of PVL SD rats models were established. Then they were randomly divided into intervention （ n = 40） and non - intervention groups （ n = 40）. The sham - operation rats served as control group （n = 40）. Then the enriched environment intervention was administrated to the intervention groups. Non- intervention groups and the sham- operation groups were breeded in standard environment, and did not intervent by enriched environment. On the fifth week, the abilities of sensorimotor movement （hanging test and inclined plane test）, learning and memory function were evaluated. On the 4th, 1 1th, 18th ,25th ,32th day, the expression of GAP- 43 in the hippocampus was measured by the method of reverse transcription - polynerase chain reaction （ RT - PCR）. Results The abilities of sensorimotor movement [ hanging test： （4.5 ± 1.5 ） min vs （3.1 ±0.9 ） min ; inclined plane test ： （ 2.3 ±0.7 ） s vs （ 3.9 ±1.1 ） s ] and the learning and memory ability [ escaped latent period ： （4.86 ±2.94 ） s vs （ 15.0 ±11.23 ） s ;Space learning and memory test： （62.89 ±8.69 ） % vs （43.99 ±8.29 ） % ] of intervention group were improved compared with non - intervention group（ Pa 〈 0.05 ）. And those abilities of non - intervention group reduced and was much lower than that of sham group. RT - PCR analysis showed that expression of GAP -43 on the 11th, 18th ,25th ,32th day in the hippocampus of intervention group significantly increased compared with that of non - intervention group [ （ 3.21 ±0.45 ） vs （ 2.63 ±0.32 ） ; （ 2.74 ±0.34 ） vs （ 2.47 ±0. 25 ） ; （2.14±0.29） vs（1.75±0.19）;（1.96±0.25） vs（1.63±0. 15） Pa〈0.01].Conclusions Earlyenrichedenvironmentcanimprove brain function of PVL in premature rats. The enriched environment stimulation could increase the expression of GAP -43, and it may be mechanism of the recovery of PVL in the premature rats.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第20期1570-1572,共3页
Journal of Applied Clinical Pediatrics
基金
重庆市人口和计划生育委员会科研基金项目资助(2007-60)
关键词
未成熟大鼠
白质软化病
脑室周围
早期干预
丰富环境
生长相关蛋白
premature rat
periventricular leukomalacia
early intervention
enriched environment
growth associated protein
作者简介
廖伟，男，主治医师，讲师，博士学位，研究方向为神经系统疾病。
通讯作者：赵聪敏，男，教授，硕士学位，电子信箱zhao-54@163．com。
