摘要
目的:探讨黄芪、当归对体外培养的血管平滑肌细胞(VSMC)粘着斑激酶(FAK)表达和细胞凋亡的影响。方法:应用RT-PCR、Western blot检测黄芪、当归对FAK表达的影响;应用 Greiss试剂检测 VSMC培养液中NO_2^-含量;应用流式细胞仪检测细胞凋亡及bcl-2和caspase-3表达活性。结果:黄芪、当归注射液可以显著抑制由碱性成纤维细胞生长因子所诱导的FAK表达,增加细胞培养液中的NO_2^-含量,诱导cas-pase-3表达。下调bcl-2表达,促进体外培养的VSMC凋亡。结论:黄芪、当归诱导VSMC凋亡与其促进NO合成、抑制FAK表达、诱导促凋亡基因caspase-3表达及下调抗凋亡基因bcl-2表达有关。
Objective: To explore the effect of Astragalus membranaceus (Ast) and Angelica sinensis (Ang) on focal adhesion kinase (FAK) expression and apoptosis of cultured vascular smooth muscle cells (VSMC). Methods: RT-PCR and Western blot were used to analyze the changes of FAK expression, Greiss reagent was used to measure concentration of nitric oxide (NO) in culture medium, bcl-2 and caspase-3 protein expressions and VSMC apoptosis were detected by flow cytometry. Results: Ast and Ang could significantly inhibit FAK expression induced by basic fibroblast growth factor (bFGF), increase the NO2- content in the medium, up-regulate caspase-3 expression and down-regulate bcl-2 expression as well as induce VSMC apoptosis. Conclusion: Ast and Ang could induce VSMC apoptosis by increasing NO content, inhibiting FAK expression, down-regulating apoptotic gene bcl-2 expression and up-regulating apoptogenous gene caspase-3.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2003年第3期201-203,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金(No.3997074)
河北省自然科学基金(No。301358)
关键词
黄芪
当归
粘着斑激酶
血管平滑肌细胞
VSMC
FAK
细胞凋亡
中药
Astragalus membranaceus, Angelica sinensis, focal adhesion kinase, vascular smooth muscular cells, cell apoptosis
