摘要
本文对 3头南阳牛线粒体 DNA D- loop区 91 0 bp的核苷酸序列进行了分析。结果发现 ,3头南阳牛 D- loop区的核苷酸序列有 3种单倍型。南阳牛 1、2和 3号 D- loop区的平均核苷酸变异率分别为 0 .44%、4.84%和 0 .44% ,其高变区的平均核苷酸变异率分别为 0 .81 %、7.5 7%和 0 .0 0 %。南阳牛 1号的核苷酸变异类型只有转换一种形式 ,南阳牛 2号的核苷酸变异类型有转换、颠换、插入和缺失四种形式 ,南阳牛 3号的核苷酸变异类型有转换和插入两种形式。在 3头牛的核苷酸变异类型中 ,均以转换最常见。说明南阳牛 mt DNA D- loop区表现出丰富的核苷酸变异多态性。从线粒体D- loop区核苷酸序列的 3种单倍型分析 ,提示南阳牛可能有两种不同的母系起源。
The mitochondrial DNA D loop region of Nanyang cattle was analyzed in this study. The results showed that there were three types of nucleotide haplotype of mtDNA D loop region in experimented Nanyang cattle. The average nucleotide mutational rates of the D loop region in Nanyang cattle No.1, No.2 and No.3 were 0.44%, 4.84% and 0.44%,respectively, and their average nucleotide mutational rate of hypervariable region was 0.81%, 7.57% and 0.00%,respectively. Only transition was found in mtDNA D loop region in Nanyang cattle No.1, transition and insertion were found in cattle No.3, and four types of nucleotide variation including transition, transversion, insertion and deletion were found in cattle No.2, of which the transition was frequent. Those indicated that rich nucleotide variational polymorphism of mtDNA D loop region existed in Nanyang cattle. After analyzing the bovine mtDNA D loop nucleotide haplotypes, it was possible that there were two maternal origins in Nanyang cattle population.
出处
《黄牛杂志》
2003年第1期11-15,共5页
Journal of Yellow Cattle Science
基金
教育部留学归国人员启动基金
国家高技术研究发展计划 (86 3计划 )项目 (2 0 0 1AA2 4 30 5 2 )
国家自然科学基金 (30 0 70 5 5 1)资助
