摘要
为了探究硫酸软骨素合成酶1基因(Ch Sy-1)在马氏珠母贝中的生物矿化功能,进行了组织分布实验、缺刻实验以及RNA干扰试验。结果表明:1)Ch Sy-1基因在马氏珠母贝外套膜不同部位均有表达,其在外套膜边缘部分表达量最高;2)在不同发育时期,Ch Sy-1基因在极体期、2细胞期和32细胞期表达量较高:3)缺刻48 h和72 h后,Ch Sy-1基因在外套膜的表达量明显上升;4)干扰BMP2基因后,BMP2表达受抑制而Ch Sy-1的表达量上升,这与Ch Sy-1基因在BMP信号通路中与BMP呈负相关的作用机理相一致。这表明Ch Sy-1在马氏珠母贝的生物矿化中起重要作用,为阐述其在马氏珠母贝中的生物矿化功能提供了重要资料。
In order to investigate the mechanism of chondroitin sulfate synthase 1( Ch Sy-1) participating in biomineralization in the pearl oyster Pinctada fucata,we performed tissue distribution experiment,shell notching experiment and RNA interference test. Results showed that Ch Sy-1 gene was expressed in different parts of the mantle of P. fucata,,highly in the edge of the mantle. At the different developmental stages,the expression of Ch Sy-1 was higher at the polar body phase,2-cell stage and 32-cell stage. The results of shell notching experiment showed that the expression of Ch Sy-1 gene increased significantly after 48 and 72 hours,and that the expression level of Ch Sy-1 also increased significantly after knockout of BMP2 gene,which is consistent with the mechanism by which Ch Sy-1 plays a negative regulatory role in the BMP signaling pathway. This study shows that Ch Sy-1 plays an important role in the biomineralization of P. fucata,which provides important information for the characterization of Ch Sy-1 in the biomineralization of P. fucata.
出处
《热带生物学报》
2017年第4期377-382,共6页
Journal of Tropical Biology
基金
广东省渔业科技与发展专项(Z2014014
Z2015014)
广东省科技计划项目(2014B030301064)
