摘要
为了筛选适合用于冷冻保存小鼠体外受精2细胞期胚胎的方法,本研究采用EFS20/40法和GP25法对体外受精小鼠2细胞胚进行冷冻保存,并经解冻后进行体外培养,观察其胚胎发育至扩张囊胚的能力。其结果,虽然两种方法冷冻解冻后的正常胚胎回收率相近,但采用EFS20/40法冷冻解冻的胚胎在体外发育至扩张囊胚比率(84.9%)显著高于GP25法的54.8%(P<0.05)。表明EFS20/40法适合用于小鼠体外受精2细胞期胚胎的冷冻保存。
2 cell stage embryos in vitro fertilization from mouse were vitrified using EFS20/40,and Schefen-GP25 methods followed by thawing the embryos to evaluate the ability of expanded blastocysts to filter the better suitable vitrification scheme. The results showed that the blastocyst formation rate by EFS20/40 method（ 84.9%） was significantly higher than those by Schefen-GP25 methods（ P 0. 05） after vitrication-thawing 2-cell embryos,though the recovery rate of normal embryos is similar. These results indicated that EFS20/40 method was the better suitable for the 2-cell stage embryos in vitro fertilization from mice cryopreservation.
出处
《黑龙江动物繁殖》
2017年第6期3-5,共3页
Heilongjiang journal of animal reproduction
基金
黑龙江自然基金项目(C2016012)
关键词
小鼠
体外受精
2细胞期胚
玻璃化冷冻
mouse
in vitro fertilization
2 - cell - stage - embryo
vitrification
作者简介
朴香丽（1975-）,女,高级兽医师.E-mail：hljd4220144@163.com;通信作者：朴善花,女,高级畜牧师.E-mail：antiezhu@qq.com
