摘要
【目的】探究满天星(Gypsophila paniculata L.)组培苗在缺铁胁迫条件下的生理响应机制,为满天星组培苗的铁营养调控及培养基优化提供理论依据。【方法】以满天星组培苗为研究材料,设置不同浓度缺铁(0、10、50µmol/L Fe^(2+))处理。观察不同处理下组培苗表型及其生长指标的变化,同时测定叶绿素荧光动力学曲线及820 nm光反射曲线。【结果】短期(14 d)缺铁(10µmol/L Fe^(2+))培养有利于株高增长,但长期(35 d)缺铁(0~10µmol/L Fe^(2+))可导致满天星组培苗幼叶黄化发白,叶绿素含量显著降低(P<0.05)。叶绿素荧光分析结果显示:缺铁(0~10µmol/L Fe^(2+))导致PSⅡ供体侧和受体侧(QA→QB)电子传递受阻,单位活性反应中心数量(RC/CSO)减少、能量捕获(TRO/RC)和耗散(DIO/RC)增加,PSⅠ末端电子受体还原能力下降,且质体醌池容量减少;PSⅡ最大光化学效率(φPo)、性能指数(PIabs)和总性能指数(PItotal)下降;TRO/RC、DIO/RC等参数可作为指示缺铁胁迫的关键参数。50µmol/L Fe^(2+)处理可维持满天星组培苗正常发育和光合电子传递。【结论】培养基内铁(Fe^(2+))浓度低于10µmol/L会抑制PSⅠ/PSⅡ活性与电子传递,抑制满天星组培苗生长发育;50µmol/L Fe^(2+)处理可满足满天星组培苗正常生长和光合需求,可作为组培生产中的标准化铁营养供给基准。研究结果可为满天星组培苗培养基优化和铁营养管理提供关键阈值参数和理论依据。
[Purpose]To investigate the physiological response mechanisms of Gypsophila panicu-lata L.tissue-cultured seedlings under iron deficiency stress conditions,providing a theoretical basis for iron nutrition regulation and culture medium optimization of G.paniculata tissue-cultured seed-lings.[Methods]Using G.paniculata tissue-cultured seedlings as experimental material,iron defi-ciency treatments with different concentrations(0,10,50µmol/L Fe^(2+))were applied.Phenotypic and growth index changes under different treatments were observed,and chlorophyll fluorescence kinet-ics curves and 820 nm light reflectance curves were measured.[Results]Short-term(14 d)iron de-ficiency(10µmol/L Fe^(2+))treatment promoted plant height growth,whereas long-term(35 d)iron defi-ciency(0-10µmol/L Fe^(2+))induced chlorosis and whitening in young leaves of the seedlings,accom-panied by a significant reduction in chlorophyll content(P<0.05).Chlorophyll fluorescence analysis revealed that iron deficiency(0-10µmol/L Fe^(2+))disrupted electron transport on both the donor side and acceptor side(QA→QB)of PSⅡ,reduced the number of active reaction centers per cross-section(RC/CSO),increased energy capture(TRO/RC)and dissipation(DIO/RC),decreased the reducing capa-city of terminal electron acceptors in PSⅠ,and diminished the plastoquinone pool capacity.Concur-rently,the maximum photochemical efficiency(φPo),performance index(PIabs),and total perform-ance index(PItotal)of PSⅡwere reduced.Parameters such as TRO/RC and DIO/RC were identified as key indicators for iron deficiency stress.The 50µmol/L Fe^(2+)treatment maintained normal seedling development and photosynthetic electron transport.[Conclusion]Iron(Fe^(2+))concentrations below 10µmol/L in the culture medium inhibit PSⅠ/PSⅡactivity and electron transport,thereby impairing the growth of G.paniculata tissue-cultured seedlings.The 50µmol/L Fe^(2+)treatment meets the re-quirements for normal growth and photosynthetic function in tissue-cultured seedlings,and can serve as the standardized benchmark for iron nutrition supply in tissue culture production.These findings provide critical threshold parameters and theoretical basis for optimizing the culture medium and managing iron nutrition in G.paniculata tissue culture.
作者
吴杰
刘镕
王玲
SAUBAN MUSA Jibri
李娅娟
李成云
WU Jie;LIU Rong;WANG Ling;SAUBAN MUSA Jibril;LI Yajuan;LI Chengyun(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Kunming 650201,China)
出处
《云南农业大学学报(自然科学版)》
北大核心
2025年第4期100-110,共11页
Journal of Yunnan Agricultural University:Natural Science
基金
国家重点研发计划项目(2023YFD1400800)。
关键词
满天星组培苗
缺铁胁迫
叶绿素荧光
JIP-test分析
Gypsophila paniculata tissue-cultured seedlings
iron deficiency stress
chlorophyll fluor-escence
JIP-test analysis
作者简介
吴杰(1998-),男,贵州遵义人,在读硕士研究生,主要从事分子植物病理学研究。E-mail:1436976424@qq.com;通信作者:李成云(1964-),男,云南昆明人,学士,教授,主要从事植物病原菌的致病机制和真菌病害生态防控研究。E-mail:lichengyun@ynau.edu.cn。
