摘要
目的了解山西省中部及南部地区鼠类伯氏疏螺旋体、问号钩端螺旋体、莫氏立克次体和恙虫病东方体的携带情况,为当地鼠传疾病的防控提供参考依据。方法2024年7月,采用夹夜法在山西省晋中市、阳泉市及运城市的7个镇(乡)捕获鼠类,无菌收集其肝脏、脾脏和肾脏组织,采用荧光定量PCR法对4种病原体进行检测。以χ^(2)检验或Fisher确切概率法比较不同鼠种、性别、组织、生境和采样点的病原体检出率差异。结果捕获黑线姬鼠、苛岚绒鼠、中华姬鼠、小家鼠、北社鼠、长尾仓鼠、黄胸鼠和大仓鼠8种鼠类共301只。采用实时荧光定量PCR对301只鼠的肝、脾和肾脏标本进行伯氏疏螺旋体、问号钩端螺旋体、莫氏立克次体和恙虫病东方体检测。结果显示伯氏疏螺旋体、问号钩端螺旋体检出率分别为16.94%(51/301)、3.99%(12/301),莫氏立克次体和恙虫病东方体未检出。伯氏疏螺旋体的检出率高于问号钩端螺旋体(χ^(2)=117.430,P<0.01)。伯氏疏螺旋体在中华姬鼠、黑线姬鼠、小家鼠和北社鼠4种鼠类检出,问号钩端螺旋体在黑线姬鼠、岢岚绒鼠、小家鼠和北社鼠4种鼠类检出,伯氏疏螺旋体在肾脏组织中检出率最高,为13.62%,高于肝脏和脾脏中的检出率(χ^(2)=35.999,P<0.01);问号钩端螺旋体三种组织中的检出率差异均无统计学意义(χ^(2)=3.426,P=0.180)。伯氏疏螺旋体和问号钩端螺旋体在村庄生境捕获的2只鼠中未检出,在林区、田地及退耕林地捕获鼠均被检出。伯氏疏螺旋体在退耕林地检出率最高(21.28%),问号钩端螺旋体在田地中检出率居首(7.50%)。结论山西省中部及南部地区鼠类存在伯氏疏螺旋体和问号钩端螺旋体感染,提示应开展人群感染调查。
Objective To investigate the prevalence of Borrelia burgdorferi,Leptospira interrogans,Rickettsia mooseri,and Orientia tsutsugamushi in rodents in the central and southern regions of Shanxi Province,thereby providing a reference for the prevention and control of local rodent-borne diseases.Methods In July 2024,rodents were captured using the night trapping method in 7 towns(townships)of Jinzhong,Yangquan,and Yuncheng Cities,Shanxi Province.The liver,spleen,and kidney tissues of rodents were collected under aseptic conditions for the detection of the above four pathogens by fluorescence quantitative PCR(qPCR).Chi-square or Fisher's exact tests were employed to compare pathogen-positive rates among different species,sexes,tissues,habitats,and sampling sites.Results In total,301 rodents belonging to 8 species were captured,including Apodemus agrarius,Eothenomys inez,Apodemus draco,Mus musculus,Niviventer confucianus,Cricetulus longicaudatus,Rattus tanezumi,and Tscherskia triton.Real-time fluorescent quantitative PCR screening of liver,spleen,and kidney specimens of 301 rodents showed overall positivity rates of 16.94%(51/301)for B.burgdorferi,3.99%(12/301)for L.interrogans,and 0 for both R.mooseri and O.tsutsugamushi.The detection rate of B.burgdorferi was higher than that of L.interrogans(χ^(2)=117.430,P<0.01).B.burgdorferi was detected in four species of rodents,namely Apodemus draco,Apodemus agrarius,Mus musculus,and Niviventer confucianus.L.interrogans was detected in four species of rodents,including Apodemus agrarius,Eothenomys inez,Mus musculus,and Niviventer confucianus.Among tissues,kidneys showed the highest detection rate for B.burgdorferi(13.62%),significantly higher than those of liver and spleen(χ^(2)=35.999,P<0.01).There was no significant difference in the detection rates of L.interrogans among the three tissues(χ^(2)=3.426,P=0.180).No pathogens were detected in the two rodents captured in the village habitat.Both pathogens were found in rodents from forest,farmland,and reforested cropland,with the highest B.burgdorferi positivity in reforested areas(21.28%)and the highest L.interrogans positivity in farmland(7.50%).Conclusion Rodents in central and southern Shanxi Province are infected with B.burgdorferi and L.interrogans,indicating potential infection risks to the local human population.Relevant public-health authorities should pay close attention to these findings.
作者
牛靖蓉
刘益萍
程红兵
任飞
赵超
崔佳
白育瓒
赵雨馨
栗冬梅
于娟
饶华祥
NIU Jingrong;LIU Yiping;CHENG Hongbing;REN Fei;ZHAO Chao;CUI Jia;BAI Yuzan;ZHAO Yuxin;LI Dongmei;YU Juan;RAO Huaxiang(Department of Basic Medical Sciences,Changzhi Medical College,Changzhi,Shanxi 046000,China;Department of Laboratory Animal Center,Changzhi Medical College,Changzhi,Shanxi 046000,China;Department of Public Health and Preventive Medicine,Changzhi Medical College,Changzhi,Shanxi 046000,China;Shanxi Higher Education Institutions of Science and Technology Innovation Plan Platform,Laboratory of Environmental Factors and Population Health,Changzhi,Shanxi 046000,China;Key Laboratory of Environmental Pathogenic Mechanisms and Prevention of Chronic Diseases,Changzhi Medical College,Changzhi,Shanxi 046000,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Department of Vector Biology and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《中国热带医学》
北大核心
2025年第8期1011-1015,共5页
China Tropical Medicine
基金
山西省基础研究计划项目(No.202303021221180)
山西省高等学校科技创新计划项目(No.2024L291)
山西省大学生创新创业训练项目(No.20240988)。
关键词
鼠类
鼠传病原体
伯氏疏螺旋体
问号钩端螺旋体
中华姬鼠
黑线姬鼠
Rodents
rodent-borne pathogens
Borrelia burgdorferi
Leptospira interrogans
Apodemus draco
Apodemus agrarius
作者简介
牛靖蓉(1996-),女,硕士,助理实验师,研究方向:自然疫源性疾病防控;通信作者:饶华祥,E-mail:raohuaxiang2006006@163.com。
