摘要
目的:探究牛血清白蛋白(BSA)与槲皮素(Que)、黄芩素(Bai)、漆黄素(Fis)的结合机制及BSA对黄酮的保护作用。方法:采用荧光光谱法结合分子对接探究Que、Bai、Fis与BSA的结合机制,分析3种黄酮能否实现在BSA上的共装载。根据保留率、清除率、抑制率等指标探究BSA对3种黄酮热稳定性、紫外稳定性、抗氧化性、胰脂肪酶抑制活性的影响。结果:Que依靠疏水相互作用,Bai、Fis通过静电相互作用与BSA结合形成复合物,结合常数分别为5.27×10^(6),1.00×10^(5),1.20×10^(6) L/mol,结合位点数均为1个。位点Marker试验和分子对接显示,Que和Bai结合在BSA的ⅠB和ⅢA之间,Fis结合在ⅡA附近。BSA可同时结合3种黄酮,形成BSA-Que-Bai-Fis和BSA-Que-Fis-Bai。与BSA的结合可以改善黄酮的稳定性和活性。对Que而言,在热稳定性、紫外稳定性、抗氧化活性和胰脂肪酶抑制活性方面,BSA-多配体复合物均比BSA-单配体复合物的保护效果好,保留率/清除率/抑制率最高可达(31.30±2.60)%,(19.69±1.30)%,(55.46±2.20)%,(55.21±0.86)%。对Bai而言,在热稳定性、紫外稳定性上BSA-单配体复合物比BSA-多配体复合物的保护作用好,保留率最高可达(71.20±2.30)%,(64.11±1.40)%,而在抗氧化和胰脂肪酶抑制活性方面,BSA-多配体复合物的效力更好。Fis的情况与Que相同,经热处理、紫外处理后保留率最高可达(61.16±2.00)%,(59.37±0.94)%。结论:BSA可以保护黄酮,是一种有效的天然活性成分保护载体。
Objective:To investigate the binding mechanism of bovine serum albumin(BSA)with quercetin(Que),baicalein(Bai),and fisetin(Fis),as well as the protective effect of BSA on flavonoids.Methods:Fluorescence spectroscopy combined with molecular docking was used to explore the binding mechanism between Que,Bai,Fis and BSA,and to analyze whether the three flavonoids can achieve co loading on BSA.Exploring the effects of BSA on the thermal stability,UV stability,antioxidant activity,and pancreatic lipase inhibition activity of three flavonoids was based on retention rate,clearance rate,inhibition rate,and other indicators.Results:Que relied on hydrophobic interactions to bind to BSA,while Bai and Fis bind to BSA through electrostatic interactions to form complexes.The binding constants were 5.27×10^(6),1.00×10^(5) L/mol,and 1.20×10^(6) L/mol,respectively,and the number of binding sites was 1.The site marker assay and molecular docking showed that Que and Bai bind betweenⅠB andⅢA of BSA,while Fis binds nearⅡA.BSA could simultaneously bind to three flavonoids to form BSA-Que-Bai-Fis and BSA-Que-Fis-Bai.Binding with BSA could improve the stability and activity of flavonoids.For Que,BSA-multiligand complexes had better protective effects than BSA-monoligand complexes in terms of thermal stability,UV stability,antioxidant activity,and pancreatic lipase inhibition activity.The highest retention rate/clearance rate/inhibition rate could reach(31.30±2.60)%,(19.69±1.30)%,(55.46±2.20)%,and(55.21±0.86)%.For Bai,BSA-monoligand complexes had better protective effects than BSA-multiligand complexes in terms of thermal stability and UV stability,with retention rates up to(71.20±2.30)%and(64.11±1.40)%,respectively.In terms of antioxidant and pancreatic lipase inhibitory activity,BSA-multiligand complexes had better efficacy.The situation of Fis was the same as Que,with the highest retention rates of(61.16±2.00)%and(59.37±0.94)%after heat treatment and UV treatment.Conclusions:BSA can protect flavonoids and is an effective natural carrier for protecting active ingredients.
作者
张璇
王昆山
周素珍
范金波
ZHANG Xuan;WANG Kunshan;ZHOU Suzhen;FAN Jinbo(College of Food Science and Engineering,Bohai University,Jinzhou 121013,Liaoning)
出处
《中国食品学报》
北大核心
2025年第8期76-90,共15页
Journal of Chinese Institute of Food Science and Technology
关键词
槲皮素
黄芩素
漆黄素
牛血清白蛋白
保护
稳定性
活性
quercetin(Que)
baicalein(Bai)
fisetin(Fis)
bovine serum albumin(BSA)
protection
stability
activity
作者简介
第一作者:张璇,女,硕士生;通信作者:范金波,E-mail:jinbo_fan@hotmail.com。
