摘要
目的:探讨含纤维蛋白原C结构域1(FIBCD1)在肺癌中的作用。方法:采用慢病毒介导的RNA干扰技术构建FIBCD1敲低的A549肺癌细胞株,通过实时定量PCR和蛋白质印迹法验证敲低效率。利用MTT法评估细胞增殖情况,流式细胞术确定细胞周期和凋亡情况,菌落形成及动物成瘤试验检测敲低FIBCD1对A549细胞菌落形成及肿瘤生长的影响。结果:FIBCD1敲低显著抑制A549细胞增殖,诱导G0/G1期细胞周期阻滞,并显著提高细胞凋亡率。菌落形成试验进一步证实,抑制FIBCD1会影响A549细胞的菌落形成。动物实验结果显示,敲低FIBCD1显著减缓裸鼠体内肿瘤的生长,肿瘤质量与体内发光强度均低于对照组。结论:FIBCD1在肺癌细胞中具有促进增殖、抑制凋亡的作用,其高表达可能与肿瘤生长密切相关。FIBCD1的抑制可显著降低肺癌细胞的致瘤能力,提示其有望作为肺癌治疗的潜在靶点。
Objective:To explore the role of fibrinogen-related C domain 1(FIBCD1)in lung cancer.Methods:A FIBCD1-knockdown A549 lung cancer cell line was constructed using lentivirus-mediated RNA interference.The knockdown efficiency was validated by quantitative real-time PCR and Western blotting.Cell proliferation was assessed by MTT assay,while cell cycle distribution and apoptosis were analyzed via flow cytometry.The effects of FIBCD1 knockdown on colony formation and tumor growth were evaluated using colony formation assay and in vivo xenograft tumor formation assay in nude mice.Results:FIBCD1 knockdown significantly inhibited A549 cell proliferation,induced G0/G1 phase cell cycle arrest,and markedly increased the apoptotic rate.Colony formation assay further confirmed that FIBCD1 suppression impaired the colony-forming ability of A549 cells.In vivo experiments showed that FIBCD1 knockdown significantly attenuated tumor growth in nude mice,with reduced tumor weight and in vivo luminescence intensity compared to the control group.Conclusion:FIBCD1 exerts a role in promoting proliferation and inhibiting apoptosis in lung cancer cells.Its high expression may be closely associated with tumor growth.Suppression of FIBCD1 significantly reduces the tumorigenic potential of lung cancer cells,indicating that FIBCD1 holds promise as a potential therapeutic target for lung cancer.
作者
李华
张敏
方佩斐
张燕飞
Li Hua;Zhang Min;Fang Peifei;Zhang Yanfei(School of Medical Science,Anhui Institute of Medicine,Hefei 230601,China)
出处
《大理大学学报》
2025年第8期51-56,共6页
Journal of Dali University
基金
安徽医学高等专科学校高层次人才科研启动基金项目(2024RC008)。
关键词
含纤维蛋白原C结构域1
流式细胞术
菌落形成
动物成瘤试验
fibrinogen-related C domain 1
flow cytometry
colony formation
in vivo xenograft tumor formation assay
作者简介
第一作者:李华,讲师,主要从事解剖生理研究。
