摘要
为了建立一种高效且精准的食品中酚类防腐剂及抗氧化剂检测方法,将市售大豆油作为检测对象,采用高效液相色谱法对富马酸二甲酯、没食子酸丙酯、2,6-二叔丁基对甲基苯酚、叔丁基对羟基苯酚及叔丁基对苯二酚5种目标化合物展开系统检测。结果表明,目标化合物在质量浓度为0.5~100.0 mg·L^(-1)时线性关系良好(相关系数大于0.996),最低检出限为0.3~1.5 mg·kg^(-1),加标回收率为89.12%~97.45%,相对标准偏差小于6%;实际样品检测仅检出微量2,6-二叔丁基对甲基苯酚(0.18~0.25 mg·kg^(-1)),符合国家标准。该方法适用于复杂油脂基质中痕量酚类添加剂的精准测定,为食品安全监管提供了可靠的技术支持。
In order to establish an efficient and accurate detection method for phenolic preservatives and antioxidants in food,commercially available soybean oil is taken as the detection object,five target compounds,namely dimethyl fumarate,propyl gallate,2,6-di-tert-butyl-p-methylphenol,tert-butyl-p-hydroxyphenol and tertbutylhydroquinone,are systematically detected by high performance liquid chromatography.The results show that the linear relationship of the target compound is good within the mass concentration range from 0.5 mg·L^(-1) to 100.0 mg·L^(-1)(correlation coefficient greater than 0.996),the minimum detection limit is from 0.3 mg·L^(-1) to 1.5 mg·kg^(-1),the spiked recovery rate is from 89.12%to 97.45%,and the relative standard deviation is less than 6%.The actual sample test only detected trace amounts of 2,6-di-tert-butyl-p-methylphenol(0.18 mg·kg^(-1) to 0.25 mg·kg^(-1)),which conformed to the national standard.This method is applicable to the precise determination of trace phenolic additives in complex lipid matrices,providing reliable technical support for food safety supervision.
作者
张薇
ZHANG Wei(Tianjin Beichen District Center for Disease Control and Prevention(Tianjin Beichen District Health Supervision Institute),Tianjin 300400,China)
出处
《食品安全导刊》
2025年第24期67-70,共4页
Food Safety Guide Magazine
关键词
高效液相色谱法
酚类防腐剂
抗氧化剂
大豆油
固相萃取
high performance liquid chromatography
phenolic preservatives
antioxidant
soybean oil
solid phase extraction
作者简介
张薇(1986-),女,天津人,本科,工程师。研究方向:食品安全风险监测。
