摘要
为探索出一种准确、高效、适用的辣椒花药培养再生植株的倍性鉴定方法,以来自10个基因型的60个辣椒花药培养再生株系为试材,采用植株育性检测法和DNA流式细胞仪检测法,并结合根尖细胞压片染色体计数法进行倍性鉴定,比较不同方法的优缺点。结果表明,DNA流式细胞仪倍性鉴定准确率为96.67%,该方法快速,便捷,但鉴定成本高,且易出现偏峰现象;花粉活力检测法在判断再生植株染色体奇偶倍性方面准确率为100%,但不能甄别再生植株的具体染色体倍性。花粉活力检测法和DNA流式细胞仪检测法结合使用可明显降低鉴定成本,提高准确率和效率,即对已现蕾的株系,采用花粉活力检测法确定其染色体奇偶倍性,便于早期进行染色体加倍处理;对现蕾较晚的株系,宜在苗期采用DNA流式细胞仪检测法。
In order to explore an accurate,efficient,and applicable ploidy identification method for regenerated plants from pepper anther culture,in this study,60 regenerated plant lines from 10 genotypes of pepper anther culture were used as the test materials.The ploidy identification was conducted by adopting the plant fertility detection method and DNA flow cytometry,combined with the chromosome counting method of root tip cell squash.The advantages and disadvantages of different methods were compared.The results indicated that the accuracy rate of ploidy identification by DNA flow cytometry was 96.67%,this method was rapid and convenient,but it had high cost and was prone to peak bias.The pollen vitality detection method had an accuracy rate of 100%in determining the odd-even ploidy of regenerated plants'chromosomes,but it could not distinguish the specific ploidy of regenerated plants.The combination of pollen vitality detection method and DNA flow cytometry detection method could significantly reduce the identification cost,improve the accuracy rate and efficiency.That is,for the lines that have already flowered,the pollen vitality detection method could be used to determine the odd-even ploidy of chromosomes,which was convenient for early chromosome doubling treatment;for the lines that flowered later,the DNA flow cytometry detection method should be used in the seedling stage.If there were difficult-to-determine situations such as peak bias,the pollen vitality could be combined to determine the ploidy of regenerated plants.
作者
程志芳
李丽
史艳艳
韩娅楠
常晓轲
姚秋菊
CHENG Zhifang;LI Li;SHI Yanyan;HAN Yanan;CHANG Xiaoke;YAO Qiuju(Institute of Vegetable,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)
出处
《中国瓜菜》
北大核心
2025年第8期77-82,共6页
China Cucurbits And Vegetables
基金
河南省重大科技专项(241100110200)
河南省现代农业产业技术体系项目(CAR-24-G-15)
河南省农业科学院自主创新项目(2024ZC036)
河南省科技攻关项目(252102110318)。
关键词
辣椒
倍性鉴定
流式细胞仪
花粉活力
染色体计数法
Pepper
Ploidy identification
Flow cytometer
Pollen viability
Chromosome counting
作者简介
程志芳,女,助理研究员,主要从事茄果类蔬菜生物技术研究。E-mail:czhifang2021@163.com;通信作者:姚秋菊,女,研究员,主要从事茄果类蔬菜栽培与育种研究。E-mail:wgqdaisy@163.com。
