摘要
在前期研究基础上,以四倍体组培苗为对照,利用组培扦插的方式扩繁苜蓿单倍体植株,筛选适合单倍体炼苗移栽的营养土,并对移栽成活的单倍体植株进行自交授粉,观测其生长和结荚情况。结果显示,新疆大叶苜蓿二倍体植株组培扦插适宜的生根培养基为1/2 MS+0.1mg/L NAA+2%蔗糖+0.7%琼脂,生根率可达83%。进行炼苗移栽时,采用无菌营养土可使二倍体成活率达41.7%。苜蓿二倍体植株自交结实率和种子活力指数均显著高于四倍体。
In order to rapidly propagate haploid plants and solve the problem that it is difficult for alfalfa haploid to survive after transplanting.On the basis of previous research,the tetraploid tissue culture seedlings of alfalfa were used as the control,the haploid were propagated by tissue culture cutting,the nutrient soil suitable for haploid hardening-seedling transplanting was screened,and the transplanted haploid plants were self-pollinated to observe their growth and pod fermentation.The results showed that the suitable rooting medium for diploid cuttings of Medicago sativa L.cv.Xinjiang Daye was 1/2 MS+0.1 mg/L NAA+2%sucrose+0.7%agar,and the rooting rate was 83%.When the seedlings were transplanted,the survival rate of diploid plants could reach 41.7%by using aseptic nutrient soil.The inbred pod setting percentage and seed vigor index of alfalfa diploid plants was significantly higher than that of tetraploid plants.
作者
徐舶
石凤翎
高霞
乌日娜
杨艳婷
杨婕
王芬
XU Bo;SHI Feng-ling;GAO Xia;WU Ri-na;YANG Yan-ting;YANG Jie;WANG Fen(College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010011,China)
出处
《中国草地学报》
CSCD
北大核心
2019年第6期67-71,115,共6页
Chinese Journal of Grassland
基金
国家自然科学基金项目(31402126,31260576)
内蒙古自治区应用技术研究与开发资金计划(201702072)
草地资源教育部重点实验室内蒙古农业大学科技专项(YZGC2017008)
内蒙古自治区草原英才“内蒙古高原乡土草种质资源遗传改良与利用”创新团队
草地资源教育部重点实验室.
关键词
单倍体
新疆大叶苜蓿
组培扦插
炼苗移栽
自交结实率
Haploid
Medicago sativa L.cv.Xinjiang Daye
Tissue culture cutting
Seedling transplanting
Inbred pod setting percentage
作者简介
徐舶(1990-),女,辽宁沈阳人,在读博士研究生,研究方向为牧草遗传育种,E-mail:xubo19900121@126.com;通讯作者:石凤翎,E-mail:sfl0000@126.com
