摘要
本试验旨在研究饲料蛋氨酸(Met)水平对大口黑鲈(Micropterus salmoides)稚鱼生长性能、消化和免疫抗氧化能力的影响。选取初始体质量为(0.18±0)g的大口黑鲈1440尾,随机分为6组(每组4个重复,每个重复60尾),饲养于室外养殖系统水体体积为50 L的尼龙网箱中,分别饲喂T1~T6(蛋氨酸添加量分别为0、0.5%、1.0%、1.5%、2.0%和2.5%,实测值分别为0.67%、1.08%、1.43%、1.78%、2.22%和2.61%)6种等脂等氮饲料,试验期为30 d。结果表明:随着饲料蛋氨酸水平升高,试验鱼增重率、特定生长率、蛋白质沉积率和脂肪沉积率先升高后降低,饲料系数先降低后升高,T2、T3、T4、T5和T6组的增重率、特定生长率均显著高于T1组(P<0.05),饲料系数显著低于T1组(P<0.05);随着饲料Met水平升高,试验鱼肠道胰蛋白酶活性呈先上升后下降趋势,T4组最高,对其他消化酶活性则无明显影响;试验鱼肝脏谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)、溶菌酶(LZM)活性先升高后降低,CAT、T-SOD、LZM活性在T4组时最高,显著高于T1组(P<0.05)。饲料中适量添加Met能促进大口黑鲈稚鱼生长、消化吸收能力,提高大口黑鲈稚鱼免疫抗氧化能力,以SGR和抗氧化指标GSH-Px为评价指标获得大口黑鲈稚鱼蛋氨酸需要量分别为饲料干质量的1.72%和1.51%。
This experiment was conducted to study the effects of dietary methionine levels on the growth performance,digestion and absorption related indexes and antioxidant capacity of juvenile Micropterus salmoides.1440 largemouth bass with an initial body weight of(0.18±0)g were selected and randomly divided into 6 groups(each group had 4 replicates,with 60 replicates each).They were raised in nylon cages with a water volume of 50 L in an outdoor aquaculture system,and fed with equal nitrogen and fat feed ranging from T1 to T6(methionine additions of 0,0.5%,1.0%,1.5%,2.0%and 2.5%,with measured values of 0.67%,1.08%,1.43%,1.78%,2.22%and 2.61%,respectively).The experimental period is 30 days.The results showed that as the feed methionine level increased,the weight gain rate,specific growth rate,and protein deposition of the experimental fish first increased and then decreased,and the feed coefficient first decreased and then increased.The weight gain rate and specific growth rate of the 1.08%,1.43%,1.78%,2.22%and 2.61%groups were significantly higher than the 0.67%group(P<0.05),and the feed coefficient was significantly lower than the 0.67%group(P<0.05);With the increase of feed methionine levels,the activity of trypsin in the intestinal tract of experimental fish showed a trend of first increasing and then decreasing,with the highest in the 1.78%group,and had no significant effect on other intestinal enzyme activities;With the increase of feed methionine levels,the activities of GSH-Px,CAT,T-SOD,and LZM in the liver of experimental fish first increased and then decreased.CAT,T-SOD,and LZM activities were highest at 1.78%,significantly higher than those in the 0.67%group(P<0.05).Using specific growth rate as an evaluation indicator,the demand for methionine in juvenile largemouth bass is 1.72%,accounting for 3.23%of the feed protein.
作者
李诗洋
陈晓瑛
梁浩辉
谢雨桐
胡俊茹
黄燕华
周萌
王国霞
LI Shiyang;CHEN Xiaoying;LIANG Haohui;XIE Yutong;HU Junru;HUANG Yanhua;ZHOU Meng;WANG Guoxia(Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China;Guangdong Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China;Innovative Institute of Animal Healthy Breeding,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,Guangdong,China)
出处
《上海海洋大学学报》
北大核心
2025年第4期749-759,共11页
Journal of Shanghai Ocean University
基金
广州市基础研究计划民生科技专题(202002020018)
广东省重点领域研发计划(2021B0202050002)。
关键词
蛋氨酸
大口黑鲈
生长性能
抗氧化
肠道发育
methionine
largemouth bass
growth performance
antioxidant
intestinal development
作者简介
李诗洋(1997-),女,硕士研究生,研究方向为水产动物营养与饲料。E-mail:crisshine@163.com;通信作者:王国霞,E-mail:wanggx78@126.com。
