摘要
为了探究碳酸氢钠(NaHCO_(3))对慢性高温胁迫下大口黑鲈(Micropterus salmoides)肠道损伤的影响,实验以大口黑鲈幼鱼(20.26±0.08) g为研究对象,分别投喂含有或不含碳酸氢钠(NaHCO_(3),5 g/kg)的饲料8周。结果表明,在慢性高温胁迫下,NaHCO_(3)组特定生长率与对照组相比无显著差异,但饲料效率、蛋白质沉积率和蛋白质效率,以及肠道淀粉酶和胰蛋白酶活性显著提高。NaHCO_(3)的添加显著提高了肠道总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)活性,并上调了cat和gpx1a基因的表达,下调了keap1的基因表达。同时,NaHCO_(3)显著下调了促炎细胞因子(il-1β、tnfα和il15)的表达,上调了抗炎细胞因子il10的表达。此外,NaHCO_(3)组大口黑鲈肠道的hsp90、hsp70和hsp60基因的表达均显著低于对照组。肠道组织学分析表明,高温胁迫引起了大口黑鲈肠道损伤,饲料中添加NaHCO_(3)显著改善了肠道组织形态,并上调了肠道zo1、jam、occludin和muc2的表达。综上所述,饲料中添加5 g/kg NaHCO_(3)通过提高肠道抗氧化能力、改善肠道炎症和肠道屏障功能来有效缓解慢性高温胁迫诱导的大口黑鲈肠道损伤。研究为改善鱼类在高温胁迫下的肠道健康提供了新的视角。
In order to investigate the effect of sodium bicarbonate(NaHCO_(3))on intestinal damage in largemouth bass(Micropterus salmoides)under chronic high-temperature stress,juvenile largemouth bass(20.26±0.08)g were fed diets with or without sodium bicarbonate(NaHCO_(3),5 g/kg)for 8 weeks.The results showed that there was no significant difference in the specific growth rate in the NaHCO_(3) group compared to the control group at chronic high temperatures.However,the NaHCO_(3) group exhibited significantly improved feed efficiency,protein deposition rate,and protein efficiency,along with a marked increase in intestinal amylase and trypsin activities.The addition of NaHCO_(3) also significantly enhanced the intestinal total antioxidant capacity(T-AOC),catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPx)activities.Furthermore,NaHCO_(3) up-regulated the expression of cat and gpx1a genes and down-regulated the gene expression of keap1.Meanwhile,the expressions of pro-inflammatory cytokines(il-1β,tnfα,and il15)were significantly down-regulated in the group,while the expression of anti-inflammatory cytokine il10 was up-regulated.In addition,the expression of hsp90,hsp70,and hsp60 genes in the intestines of largemouth bass in the NaHCO_(3) group was significantly lower than that in the control group.Histologic analysis of the intestinal tract showed that high-temperature stress induced intestinal damage in largemouth bass,and the addition of NaHCO_(3) significantly improved the intestinal tissue morphology and up-regulated the expression of intestinal zo1,jam,occludin,and muc2.In conclusion,the addition of 5 g/kg NaHCO_(3) to the feed effectively alleviated chronic high-temperature stress-induced intestinal damage in largemouth bass by improving intestinal antioxidant capacity,intestinal inflammation,and intestinal barrier function.These findings may provide a new perspective to improve the intestinal health of fish under high-temperature stress.
作者
张瑞
巫丽云
曹琨琨
金俊琰
杨云霞
张志敏
韩冬
刘昊昆
朱晓鸣
解绶启
ZHANG Rui;WU Li-Yun;CAO Kun-Kun;JIN Jun-Yan;YANG Yun-Xia;ZHANG Zhi-Min;HAN Dong;LIU Hao-Kun;ZHU Xiao-Ming;Xie Shou-Qi(Dalian Ocean University,Dalian 116023,China;State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;Henan Normal University,Xinxiang 453007,China)
出处
《水生生物学报》
北大核心
2025年第8期27-35,共9页
Acta Hydrobiologica Sinica
基金
国家重点研发计划(2022YFD2400800)资助。
作者简介
张瑞(1998-),女,硕士研究生,主要从事鱼类生理生态学研究。E-mail:zhangrui01@ihb.ac.cn;通信作者:金俊琰(1983-),女,博士,主要从事鱼类生理生态学研究。E-mail:jinjunyan@ihb.ac.cn。
