摘要
基于低聚半乳糖(galactooligosaccharides,GOS)和两歧双歧杆菌(Bifidobacterium bifidum)的合生制剂对肠道屏障功能的调节作用仍不清晰。该研究旨在利用脂多糖(lipopolysaccharide,LPS)构建小鼠肠道屏障损伤模型,探究合生制剂在改善肠道屏障功能上的协同作用及机制。小鼠被随机分为正常对照组、模型组和GOS+B.bifidum CICC^(®)10395组,检测小鼠肠道通透性、紧密连接表达、细胞因子水平、黏蛋白表达及短链脂肪酸含量。并在细胞水平探究了短链脂肪酸对屏障的调节作用。结果显示,与MC组相比,GOS+B.bifidum CICC^(®)10395处理后,小鼠血清FITC-dextean、D-乳酸和二胺氧化酶浓度降低了45.24%、30.66%和32.38%;ZO-1和ZO-2 mRNA表达量增加了1.19倍和1.67倍;TNF-α、IL-1β、IL-6和髓过氧化物酶活性降低了49.07%、54.18%、38.36%和34.69%;MUC2、MUC1、MUC4和MUC17 mRNA表达量增加了1.55~8.10倍。同时,灌胃GOS+B.bifidum CICC^(®)10395促进了双歧杆菌在肠道的定植,并产生了乙酸。在细胞模型上,乙酸增强了Caco-2细胞屏障功能,降低了巨噬细胞RAW264.7促炎细胞因子的分泌。综上所述,基于GOS两歧双歧杆菌的合生制剂能够增强肠道屏障功能,降低肠道通透性,其机制与产生的乙酸有关。
The modulatory effects of synbiotic agents composed of galactooligosaccharides(GOS)and Bifidobacterium bifidum on intestinal barrier function remain unclear.This study aimed to establish a mouse model of intestinal barrier injury using lipopolysaccharide(LPS)and to investigate the synergistic effects and underlying mechanisms of these synbiotic agents in enhancing intestinal barrier function.Mice were randomly assigned to the normal control,model,and GOS+B.bifidum CICC^(®)10395 groups.Key indicators,including intestinal permeability,tight junction protein expression,cytokine levels,mucin expression,and short-chain fatty acid content,were assessed.The regulatory role of short-chain fatty acids in barrier function was further explored at the cellular level.Compared to the model group,mice treated with GOS+B.bifidum CICC^(®)10395 exhibited significant reductions in serum FITC-dextran(45.24%),D-lactic acid(30.66%),and diamine oxidase(DAO,32.38%)concentrations.ZO-1 and ZO-2 mRNA expression levels were upregulated by 2.19-fold and 2.67-fold,respectively,whereas TNF-α,IL-1β,IL-6,and myeloperoxidase(MPO)activities decreased by 49.07%,54.18%,38.36%,and 34.69%.Moreover,MUC2,MUC1,MUC4,and MUC17 mRNA expressions increased by 2.55-fold to 9.10-fold.Furthermore,GOS+B.bifidum CICC^(®)10395 administration promoted the colonization of Bifidobacterium in the intestines and increased acetic acid production.Cellular experiments demonstrated that acetic acid enhanced the barrier function of Caco-2 monolayers and inhibited pro-inflammatory cytokine secretion in RAW264.7 macrophages.In conclusion,synbiotic preparations combining GOS and B.bifidum CICC^(®)10395 demonstrated efficacy in enhancing intestinal barrier integrity and reducing permeability,likely mediated through acetic acid-dependent mechanisms.
作者
单艺
王旻
董世荣
卞春
SHAN Yi;WANG Min;DONG Shirong;BIAN Chun(School of Food Engineering,Harbin University,Harbin 150086,China;Heilongjiang Green Food Science Research Institute,Harbin 150028,China)
出处
《食品与发酵工业》
北大核心
2025年第15期32-40,共9页
Food and Fermentation Industries
基金
黑龙江省重点研发计划指导类项目(GZ2024026)
哈尔滨学院青年博士科研启动基金项目(HUDF2021113)。
关键词
低聚半乳糖
两歧双歧杆菌
肠道屏障
乙酸
细胞模型
galactooligosaccharides
Bifidobacterium bifidum
intestinal barrier
acetic acid
cell model
作者简介
第一作者:单艺,博士,副教授;通信作者:卞春,教授,E-mail:ji3526183@126.com。
