摘要
目的研究有机磷农药敌敌畏中毒后小鼠肠黏膜屏障功能的变化及新型抗毒剂宾赛克嗪的保护作用。方法昆明小鼠65只，雌雄各半，随机分为正常对照组、敌敌畏染毒模型组、宾赛克嗪2，6，18mg／kg预防治疗组；各预防治疗组腹腔注射预防给予相应药物，10min后灌胃给了敌敌畏55mg／kg，于染毒后3h取血，使用紫外分光光度计检测血浆二胺氧化酶（DAO）活性及D-乳酸质量浓度，采用单因素方差分析定量数据。结果与正常对照组比较，模型组血浆DAO活性明显升高（P〈0．01）；与模型组比较，各预防治疗组血浆DAO活性明显下降（P〈0．01），其中6mg／kg预防治疗组效果最好；与正常对照组比较，模型组血浆D-乳酸质量浓度明显升高（P〈0．01）；与模型组比较，各预防治疗组血浆D-乳酸质量浓度明显降低（P〈0．01），并且能呈剂量依赖性降低D-乳酸质量浓度。结论敌敌畏染毒小鼠血浆DAO活性及D-乳酸质量浓度显著增高，肠黏膜屏障功能遭到破坏，宾赛克嗪能减轻肠黏膜屏障功能的损伤，具有保护作用。
Objective To investigate the change of gut-barrier function of mice in acute organophosphorus poisoning and the protection effect of benthiactzine. Method A total of 65 Kumning mice, female and male both half, were randomly assigned to five groups： normal group, DDVP poisoning（model） group, and benthiactzine 2, 6, 18 mg· kg^-1 groups. The chugs were respectively given 10 minutes before intragastric administration of DDVP at a dose of 55 mg· kg^-1. The diamine oxidase（DAO） activity and concentration of D-lactate in plasma were measured by spectrophotometric assay at 3 hours after DDVP poisoning. The variance analysis was used to deal the quantitative data. Results The DAO activity in model group significantly increased compared with normal group （P 〈0.01）. Whereas, the DAO activity in all pretreatment groups were significantly decreased compared with model group（all P 〈 0.01）. The effects of benthiactzine 6 mg·kg^-1 pretreatment group was the best of all. The concentration of D-lactate in model group was significantly increased compared with normal group （ P 〈 0.01 ）. However, the concentration of D-lactate in pretreatment groups was significantly decreased compared with model group（ all P 〈 0.01 ）. The concentration of D-lactate could be decreased dose-dependently by the administration of benthiactzine. Conclusions The diamine oxidase activity and concentration of D-lactate were significantly increased in the DDVP poisoning mice, which means the gnt-barrier function was severely damaged. And the administration of benthiactzine could alleviate the injury to the gut-barrier function.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2009年第10期1074-1076,共3页
Chinese Journal of Emergency Medicine
基金
军队特需药品保密专项课题（2009ZXJ09002-014,2009ZX09301-002）
作者简介
通信作者：邱泽武，Email：qiuzw828@sina．com．cn
通信作者：汪海，Email：wh9588@yahoo．com.cn；
