摘要
为研究促生复合菌剂对混播禾草生长及土壤特性的影响,本研究以芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)、假节杆菌属(Pseudarthrobacter)等11株优良的植物根际促生菌(PGPR)为基础材料,根据菌株溶磷、固氮、分泌植物生长激素等特性配制T_(1)、T_(2)、T_(3)、T_(4)、T_(5)、T_(6)共6组复合微生物菌剂并添加到以垂穗披碱草(Elymus nutans)、青海早熟禾(Poa qinghaiensis)和中华羊茅(Festuca sinensis)为组合的混播牧草盆栽中,测定2茬牧草生长指标及土壤化学指标。结果表明:各指标同CK(加等量空白LB培养液)相比均呈现不同程度的变化,其中,垂穗披碱草、青海早熟禾和中华羊茅株高同CK相比分别增加1.49%~15.07%、1.65%~25.89%和6.36%~11.13%,总地上生物量增加4.38%~13.60%,pH降低0.03~0.11,电导率增加2.31%~14.02%,速效磷增加12.07%~27.80%,铵态氮和硝态氮分别增加7.93%~28.45%和9.78%~142.64%;灰色关联度综合分析排序为T_(1)> T_(4)> T_(3)> T_(5)> T_(2)> T_(6)> CK,复合菌剂T_(1)促生效果均优于CK及其他处理,具有良好的推广应用潜质。
In this study,we examined the effects of composite growth-promoting microbial agents on the growth and soil properties of a mixture of three grass,using 11 strains of effective plant growth-promoting rhizobacteria(PGPR),including those in the genera Bacillus,Pseudomonas,and Pseudarthrobacter.On the basis of the phosphorus solubilization,nitrogen fixation,plant growth hormone secretion,and other characteristics of the bacterial agents,we prepared six composite bacterial formulations(T_(1),T_(2),T_(3),T_(4),T_(5),and T_(6)),which were added to a mixture of the three grasses Elymus nutans,Poa qinghaiensis,and Festuca sinensis,and measured growth indices of forage and stubble and selected chemical indices of soil.The results revealed that compared with the control treatment(equal amount of LB culture solution),all indicators showed different degrees of change,among which,the plant height of E.nutans,P.qinghaiensis.and F.sinensis increased by 1.49%to 15.07%,1.65%to 25.89%,and 6.36%to 11.13%,respectively,compared with the control;the total above-ground biomass increased by 4.38%to 13.60%;the pH declined by 0.03 to 0.11 units;the electrical conductivity increased by 2.31%to 14.02%;the available phosphorus increased by 12.07%to 27.80%;and ammonium nitrogen and nitrate nitrogen increased by 7.93%to 28.45%and 9.78%to 142.64%,respectively.Furthermore,the grey relational analysis ranking for the assessed formulations was T_(1)>T_(4)>T_(3)>T_(5)>T_(2)>T_(6)>control,thereby indicting that among these formulations,the composite microbial agent T_(1) had the best promotion effect,and thus potential for promotion and application.
作者
李双雄
姚拓
柴加丽
LI Shuangxiong;YAO Tuo;CHAI Jiali(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory for Grassland Ecosystem of Education Ministry/China−America Grassland and Animal Husbandry Sustainable Development Research Center,Lanzhou 730070,Gansu,China)
出处
《草业科学》
北大核心
2025年第6期1354-1364,共11页
Pratacultural Science
基金
甘肃省林业和草原局“高寒草地退化植被恢复与土壤改良菌剂研发与示范”项目(GSAU-TSYF-2021-011)。
关键词
复合微生物菌剂
混播牧草
土壤特性
植被生长
灰色关联分析
composite microbial agent
mixed-grass
soil characteristic
vegetation growth
gray relational analysis
作者简介
第一作者:李双雄(1997-),男,甘肃镇原人,在读硕士生,研究方向为草地微生物多样性。E-mail:2351936939@qq.com;通信作者:姚拓(1968-),男,甘肃庆阳人,教授,博士,研究方向为草地微生物多样性。E-mail:yaotuo@gsau.edu.cn。
