摘要
甘薯[Ipomoea batatas(L.)Lam.]易受多种生物和非生物胁迫影响,导致产量和品质下降,因此挖掘抗逆基因并解析其功能机制,对培育甘薯抗逆新品种、保障产业可持续发展至关重要。SAUR(small auxin up RNA)基因作为生长素信号通路的关键调控因子,在调控植物生长发育和非生物胁迫响应中具有重要作用。本研究首先克隆了IbSAUR76基因,通过烟草瞬时表达发现IbSAUR76基因编码的蛋白定位于细胞核和细胞膜。组织特异性表达分析显示,IbSAUR76基因在甘薯根、茎、叶、叶柄和茎尖组织中均有表达,其中在茎尖中的表达量最高;在60 d、75 d、90 d和105 d块根中均有表达,其中在90 d块根中的达量最高。IbSAUR76启动子序列分析发现该基因上游区域包含多个逆境和激素响应相关的顺式作用元件。进一步的qRT-PCR分析显示,IbSAUR76响应干旱和盐胁迫,为了深入探究其在甘薯中的分子机制,构建了IbSAUR76的诱饵载体,通过mating法从甘薯cDNA酵母文库中筛选获得27个与IbSAUR76相互作用的候选蛋白,并对其中两个与非生物胁迫相关的互作蛋白IbERF2及IbHOS15进行了酵母双杂交回转验证。本研究丰富了对IbSAUR76基因功能的认识,为深入解析IbSAUR76在甘薯中的功能和作用机制提供了理论基础。
Sweetpotato[Ipomoea batatas(L.)Lam.]is susceptible to various biotic and abiotic stresses that adversely affect its yield and quality.Therefore,identifying stress-responsive genes and elucidating their functional mechanisms are crucial for developing stress-resistant cultivars.As key components of auxin signaling pathways,SAUR(small auxin up RNA)genes play pivotal roles in plant growth,development,and stress adaptation.In this study,the IbSAUR76 gene was cloned and its encoded protein was demonstrated to localize to the nucleus and membrane via transient expression in tobacco.Tissue-specific expression profiling demonstrated that IbSAUR76 is ubiquitously expressed in roots,stems,leaves,petioles,and shoot tips(highest in shoot tips),as well as in storage roots at 60,75,90,and 105 days post-planting(peak at 90 days).Analysis of the IbSAUR76 promoter sequence revealed the presence of multiple cis-acting elements associated with stress and hormone responses in the upstream region of the gene.qRT-PCR analysis demonstrated that IbSAUR76 responds to both drought and salt stress treatments.To elucidate the molecular mechanisms underlying its function in sweetpotato,the IbSAUR76 bait vector was constructed for yeast two-hybrid screening against a sweetpotato cDNA library using the mating method,leading to the identification of 27 candidate interacting proteins.Two interactors IbERF2 and IbERF15,which are known to be associated with abiotic stress responses,were further validated through yeast two-hybrid reciprocal verification assays.This study enhances the understanding of the IbSAUR76 gene function and provides a theoretical foundation for in-depth dissection of its role and mechanism of action in sweetpotato.
作者
白楠楠
曹艳东
郑亚婷
罗状
赵彩良
唐锐敏
吴宇浩
武小平
贾峥嵘
陈伟
贺立恒
贾小云
BAI Nannan;CAO Yandong;ZHENG Yating;LUO Zhuang;ZHAO Cailiang;TANG Ruimin;WU Yuhao;WU Xiaoping;JIA Zhengrong;CHEN Wei;HE Liheng;JIA Xiaoyun(College of Life Sciences,Shanxi Agricultural University/Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops,Taigu 030801;College of Agriculture,Shanxi Agricultural University/Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops,Taigu 030801;Institute of Cotton Research,Shanxi Agricultural University,Yuncheng 044000;Maize Research Institute,Shanxi Agricultural University,Xinzhou 034000;Sorghum Research Institute,Shanxi Agricultural University,Jinzhong 030600)
出处
《植物遗传资源学报》
北大核心
2025年第6期1106-1118,共13页
Journal of Plant Genetic Resources
基金
中央引导地方科技发展资金项目(YDZJSX2024B007)
山西农业大学科技创新提升工程(CXGC2023051,CXGC2023074)
山西农业大学杂粮研究院项目(Z120220502)
山西省回国留学人员科研资助项目(2021-074)
忻州市科学技术局项目(20220703)
山西省基础研究计划(202403021212098)。
作者简介
第一作者:白楠楠,研究方向为植物重要功能基因的挖掘与应用,E-mail:yonuoqianjin@163.com;通信作者:贾小云,研究方向为薯类作物遗传育种,E-mail:jiaxiaoyun@sxau.edu.cn。
