摘要
为提升凉山州青贮饲料质量,挖掘适合当地的特色发酵菌株,从荞麦秸秆、全株玉米和紫花苜蓿混合青贮饲料中筛选生长速度快、产酸量高、应用潜力大的优势乳酸菌.经分离筛选出1株高产乳酸菌株R1,对其生理生化特性、菌落形态和16S rRNA序列分析,并将R1作为添加剂模拟混贮发酵30 d.结果发现其菌落表面光滑呈乳白色,为革兰氏阳菌,具有很强的糖发酵能力,经16S rRNA序列分析后将其命名为Lactiplantibacillus plantarum R1.R1在pH 7.0、37℃条件下生长最佳,对NaCl的耐受性较强,且在pH 3.0的胆汁盐中,耐受力较强.将R1菌株接种到青贮饲料中混贮发酵30 d后,与对照组相比,青贮饲料的粗蛋白、水溶性碳水化合物及乳酸含量均显著升高,pH值、中性洗涤纤维和酸性洗涤纤维含量有所降低.因此,将R1作为乳酸菌添加剂可显著提高牧草青贮料的营养品质.
To improve the quality of silage in Liangshan Yi Autonomous Prefecture and identify locally suitable characteristic fermentation strains,lactic acid bacteria with fast growth,high acid production,and strong application potential were selected from a mixed silage made of buckwheat straw,whole-corn,and alfalfa.A strain R1 of high-yield lactic acid bacterial,was isolated and its physiological and biochemical characteristics,colony morphology,and 16S rRNA sequence were analyzed.The strain R1 was used as an additive in a simulated mixed silage fermentation for 30 days.The results showed that R1 formed smooth,milky white colonies,was Gram-positive,and exhibited strong sugar fermentation ability.After 16S rRNA sequence analysis,it was identified as Lactiplantibacillus plantarum R1.R1 grew best at pH 7.0 and 37℃,showing strong tolerance to NaCl,and exhibited high resistance to bile salts at pH 3.0.After inoculating the R1 strain into silage and fermenting it for 30 days,compared with the control group,the crude protein,water-soluble carbohydrates,and lactic acid contents in the silage were significantly increased,while the pH value,neutral detergent fiber,and acid detergent fiber contents were reduced.Therefore,R1 can be used as a lactic acid bacteria additive to significantly improve the nutritional quality of grass silage.
作者
梁绍容
胥露
胡文欣
赖先军
杨飞雪
杨瑞琪
张茜
向科静
史迎欣
蒲春燕
邵欢欢
颜朗
王小燕
LIANG Shaorong;XU Lu;HU Wenxin;LAI Xianjun;YANG Feixue;YANG Ruiqi;ZHANG Qian;XIANG Kejing;SHI Yingxin;PU Chunyan;SHAO Huanhuan;YAN Lang;WANG Xiaoyan(College of Life Sciences,Sichuan Normal University,Chengdu 610101,Sichuan;Sichuan Provincial Key Laboratory for Research and Utilization of Panxi Characteristic Crops,Xichang University,Xichang 615000,Sichuan)
出处
《四川师范大学学报(自然科学版)》
2025年第5期683-692,共10页
Journal of Sichuan Normal University(Natural Science)
基金
攀西特色作物研究与利用四川省重点实验室开放基金项目(SZKF2213)。
关键词
青贮
植物乳酸杆菌
分离鉴定
青贮品质
silage
Lactiplantibacillus plantarum
isolation and identification
silage quality
作者简介
通信作者:王小燕(1985-),女,助理研究员,主要从事应用微生物研究,E-mail:1048855344@qq.com。
