摘要
【背景】水绵是养殖水体常见的有害丝状藻类,但具有生物控制水绵功能的微生物资源极其匮乏。【目的】分离鉴定高效抑水绵菌株,分析其抑藻谱、抑藻方式和代谢组学特征,丰富用于养殖水体水绵控制的菌种资源。【方法】以纤细水绵(Spirogyra gracilis)FACHB-354株作为筛选指示藻,从虾池底泥中分离高效抑水绵菌株,采用16S rRNA基因序列分析和生理生化鉴定对高效抑水绵菌株进行鉴定,并在分析高效抑水绵菌株的抑藻谱、抑藻方式的基础上,采用比较代谢组学方法解析高效抑水绵菌株的代谢组学特征。【结果】从虾池底泥中分离筛选出1株高效抑水绵菌株GT5,经16S rRNA基因序列分析和生理生化鉴定确定菌株GT5为枯草芽孢杆菌(Bacillus subtilis)。此外,菌株GT5具有广谱抑水绵活性,抑藻方式为间接抑藻。以无抑藻活性的枯草芽孢杆菌KC1的胞外代谢组为对照,菌株GT5表达量显著上调的代谢物187种,表达量显著下调的代谢物169种。这些显著差异代谢物主要富集到精氨酸和脯氨酸代谢、赖氨酸降解等20个代谢通路。其中,精氨酸生物合成、组氨酸代谢、氨基苯甲酸酯降解、嘧啶代谢、精氨酸和脯氨酸代谢等5个代谢通路影响较大,参与这5个代谢通路的显著上调的差异代谢物有4-(谷氨酰氨基)丁酸酯、邻苯二酚、6-去氢睾酮葡萄糖醛酸苷、N4-乙酰氨基丁醛、胍丁胺和4-羟脯氨酸。【结论】枯草芽孢杆菌GT5是优良的抑水绵菌株,其抑水绵活性可能与邻苯二酚、胍丁胺等潜在抑藻活性物质的产生有关。
[Background]Spirogyra is a genus of common harmful filamentous algae in aquaculture,while the microbial resources capable of controlling Spirogyra are extremely scarce.[Objective]To isolate and identify an efficient anti-Spirogyra strain and analyze its algicidal spectrum,algicidal mode,and metabolomic characteristics,enriching the strain resources for the control of Spirogyra in aquaculture.[Methods]With Spirogyra gracilis FACHB-354 as an indicator strain,the efficient anti-Spirogyra strain was isolated from the shrimp pond sediment and identified by 16S rRNA gene sequence analysis and physiological and biochemical tests.Furthermore,the comparative metabolomic method was used for metabolomic characterization after the algicidal spectrum and algicidal mode assays.[Results]An efficient anti-Spirogyra strain GT5 was isolated and identified as Bacillus subtilis.This strain had a wide algicidal spectrum against Spirogyra and showed an indirect algicidal mode.With the extracellular metabolome of B.subtilis KC1 without algicidal properties as the control,strain GT5 showed 187 up-regulated metabolites and 169 down-regulated metabolites.These differential metabolites were mainly involved in 20 metabolic pathways such as arginine and proline metabolism and lysine degradation.Among these metabolic pathways,the five significant metabolic pathways were arginine biosynthesis,histidine metabolism,degradation of aminobenzoate,pyrimidine metabolism,and arginine and proline metabolism.The significantly differential metabolites involved in the upregulation of these 5 metabolic pathways included 4-(glutamylamino)butanoate,pyrocatechol,6-dehydrotestosterone glucuronide,N4-acetylaminobutanal,agmatine,and 4-hydroxyproline.[Conclusion]B.subtilis GT5 is confirmed as an efficient anti-Spirogyra strain,and its algicidal activity is probably associated with the production of potential algicidal substances such as pyrocatechol and agmatine.
作者
滕晨皓
王会聪
王友红
曹海鹏
盖春蕾
TENG Chenhao;WANG Huicong;WANG Youhong;CAO Haipeng;GAI Chunlei(Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai 201306,China;Jiangsu Vocational College of Agriculture and Forestry,Jurong 212499,Jiangsu,China;Marine Science Research Institute of Shandong Province(National Oceanographic Center),Qingdao 266104,Shandong,China)
出处
《微生物学通报》
北大核心
2025年第5期2156-2171,共16页
Microbiology China
基金
国家虾蟹产业技术体系项目(CARS-48)
江苏农林职业技术学院科技计划(2022kj58)。
关键词
枯草芽孢杆菌
抑藻谱
抑藻方式
代谢组学特征
Bacillus subtilis
algicidal spectrum
algicidal mode
metabolomic characteristics
作者简介
滕晨皓,These authors contributed equally to this work;王会聪,These authors contributed equally to this work;Corresponding authors:CAO Haipeng,E-mail:hpcao@shou.edu.cn;Corresponding authors:GAI Chunlei,497931256@qq.com。
