摘要
由松材线虫(Bursaphelenchus xylophilus)介导引起的松材线虫病给松林带来了巨大的危害,而树干注射药剂的方法为松材线虫防控开辟了一条新的途径。为减缓松材线虫对现有的药剂产生抗药性,亟需开发新型防控药剂,其中植物源天然活性物质对松材线虫具有良好的灭杀作用。选取广西壮族自治区常见的5种植物[薇甘菊(Mikania micrantha)、马缨丹(Lantana camara)、油茶(Camellia oleifera)、苦楝(Melia azedarach)和辣蓼(Polygonum hydropiper)]作为原料获得其水提液,并采用浸渍法探究其对松材线虫的毒杀作用,分析其对松材线虫活动能力和繁殖能力的影响。结果显示5种植物水提液均对松材线虫有一定的毒杀作用,其中马缨丹在72 h处理时间和40 g·L^(-1)质量浓度下松材线虫的校正死亡率为95.05%。马缨丹对松材线虫的活动能力的抑制性最强,苦楝对松材线虫的繁殖能力抑制性最强。研究结果表明马缨丹和苦楝可作为开发松材线虫绿色防控药剂的备选材料。
Pine wilt disease,caused by Bursaphelenchus xylophilus,has done significant damage to pineforests.The trunk injection method is a new way to prevent and control B.xylophilus.It is urgent to developnew prevention and control agents that can slow down B.xylophilus's resistance to existing insecticides.Plant-derived natural active substances are a promising option as they have a strong toxic effect on B.xylophilus.This study selected five common plants(Mikania micrantha,Lantana camara,Camellia oleifera,Meliaazedarach,and Polygonum hydropiper)in the Guangxi Zhuang Autonomous Region to obtain their waterextracts.The study investigated the toxic effects of these extracts on B.xylophilus using an immersion methodand analyzed their impact on B.xylophilus's activity and reproductive capabilities.The results showed that thewater extracts from all five plants had some toxic effect on B.xylophilus.Specifically,Lantana camaraachieved a corrected mortality rate of 95.05%after 72 hours of treatment at a concentration of 40 g·L^(-1).Lantana camara exhibited the strongest inhibition of B.xylophilus's activity,while Melia azedarach showedthe strongest inhibition of B.xylophilus's reproductive capability.Research results indicate that Lantanacamara and Melia azedarach can be used to serve as potential materials for developing green control agentsagainst B.xylophilus.
作者
张育华
何子婷
罗敦
钟先龙
李轲祎
孟丽华
胡平
ZHANG Yuhua;HE Ziting;LUO Dun;ZHONG Xianlong;LI Keyi;MENG Lihua;HU Ping(Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation,School of Forestry,GuangxiUhiversity,Nanning,Guangxi 530004 China;Guangxistate-Owned Bobai Forest Farm,Yulin,Guangxi 537600 China)
出处
《热带生物学报》
2025年第3期423-429,共7页
Journal of Tropical Biology
基金
中央引导地方科技发展专项项目(桂科ZY21195019)
广西自筹经费林业科技项目(桂林科研[2022ZC]第21号)
广西大学2023年大学生创新训练计划项目(202310593462)。
关键词
松材线虫
浸渍法
绿色防控
马缨丹
苦楝
Bursaphelenchus xylophilus
Impregnation method
Green pest control
Lantana camara
Meliaazedarach
作者简介
第一作者:张育华(2000-),女,广西大学林学院2022级硕士研究生。E-mail:913734024@qq.com;通信作者:胡平(1989-),女,副教授。研究方向:森林保护。E-mail:20190084@gxu.edu.cn。
