摘要
以玉竹根茎粉末为原料,以多糖保留率和蛋白质脱除率为评价指标,考察氯仿与正丁醇体积比、Sevage试剂与多糖样品体积比、振摇时间对多糖保留和蛋白质脱除效果的影响.在单因素试验基础上,通过响应面法对玉竹多糖脱蛋白工艺进行优化.结果表明,玉竹多糖脱蛋白最佳工艺条件为V(氯仿)∶V(正丁醇)=4∶1,V(Sevage试剂)∶V(多糖样品)=1∶2,振摇时间30 min.此优化条件下,多糖保留率为76.01%,蛋白质脱除率为28.73%.
Using Polygonatum odoratum(Mill.)Druce powder as the raw material,this study investigated the effects of chloroform to butyl alcohol volume ratio,Sevage reagent to polysaccharide sample volume ratio,and shaking time on polysaccharide retention rate and protein removal rate.Based on single factor experiments,the protein removal process for polysaccharides was optimized using response surface methodology.The optimal conditions were determined as follows:V(chloroform)∶V(butyl alcohol)=4∶1,V(Sevage reagent)∶V(Polysaccharide sample)=1∶2,and shaking time of 30 min.Under these conditions,the polysaccharide retention rate reached 76.01%,and the protein removal rate was 28.73%.This optimized protocol provides a reliable approach for efficiently removing proteins while preserving polysaccharide integrity during the purification of Polygonatum odoratum(Mill.)Druce polysaccharides.
作者
章扬
谢菲
陈淑婷
杜来红
胡珂美
邹彬
王琦
成江
ZHANG Yang;XIE Fei;CHEN Shuting;DU Laihong;HU Kemei;ZOU Bin;WANG Qi;CHENG Jiang(School of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;Key Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College,Jishou 416000,Hunan China)
出处
《吉首大学学报(自然科学版)》
2025年第3期75-81,共7页
Journal of Jishou University(Natural Sciences Edition)
基金
湖南省自然科学基金资助项目(2024JJ7409)
国家大学生创新创业训练计划项目(202210531011)
吉首大学药学院创新性研究项目(2024年立项)。
关键词
玉竹多糖
脱蛋白
Sevage方法
响应面法
Polygonatum odoratum(Mill.)Druce polysaccharides
deproteinization
Sevage method
response surface
作者简介
通信作者:成江(1992-),男,湖南永州人,吉首大学药学院讲师,博士,主要从事天然产物化学生物学研究.E-mail:awb_cj@163.com.
