摘要
小麦中赭曲霉毒素A(Ochratoxin A,OTA)对其造成了严重的质量安全问题,现场快速检测OTA需要稳定可靠的设备与检测方法,现阶段预处理所需设备多、检测时间长,且易受人为干扰。该研究提出了一种一体化微流控芯片,设计各功能腔室实现同步快速预处理小麦,通过将预处理后的待测液滴加在修饰了抗体的电极上对OTA阻抗感知检测,该方法仅需约15 min。阻抗感知定量检测OTA具有良好的线性关系(R^(2)=0.98)、低检出限(0.05μg/mL)和稳定的加标回收率(88.25%~112.36%)。通过测试其他干扰毒素发现微流控联用阻抗感知法对OTA检测特异性良好,与常规预处理方法和液相色谱-质谱联用方法相比,该方法具有较好的特异性、可靠性和稳定性。该方法联用微流控芯片与阻抗感知技术,可以满足小麦等食品预处理的基本检测需求,实现快速检测小麦中的OTA,更加省时省力,为食品质量安全和病害早期监测的发展提供了一定的思路。
Ochratoxin A(OTA)can contaminate wheat,thus posing a serious food safety issue.On-site rapid detection of OTA requires stable and reliable equipment and detection methods.Current pretreatment methods require extensive equipment,involve long detection times,and are susceptible to human interference.In this study,an integrated microfluidic chip with various functional chambers was proposed to achieve synchronous,rapid pretreating of wheat.Pretreated test droplets are added to electrodes modified with antibodies for OTA detection through impedance sensing.The whole process only requires 15 minutes.Quantitative detection of OTA through impedance sensing exhibited a good linear relationship(R^(2)=0.98),low detection limit(0.05μg/mL),and stable recovery(88.25%~112.36%).Testing of other confounding toxins reveals that the combined use of the pretreatment microfluidic chip and impedance sensing has good specificity for OTA detection.The proposed method demonstrates higher specificity,reliability,and stability than conventional pretreatment methods and liquid chromatography-mass spectrometry.By combining a microfluidic chip with impedance sensing,this method meets the basic pretreatment requirements for detecting OTA in wheat and other foods and enables rapid detection of OTA.This efficient and convenient method provides insight into the development of systems for food quality and safety and early disease monitoring.
作者
吴志威
孙宗保
蒋欣容
邹小波
杨宁
WU Zhiwei;SUN Zongbao;JIANG Xinrong;ZOU Xiaobo;YANG Ning(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;The Quality Monitoring Center for Food and Strategic Reserves of Zhenjiang City,Zhenjiang 212216,China;School of Electrical and Information Engineering,Jiangsu University,Zhenjiang 212013,China)
出处
《现代食品科技》
北大核心
2025年第5期312-319,共8页
Modern Food Science and Technology
基金
国家重点研发青年科学家项目(2022YFD2000200)
江苏省农业科技自主创新项目(CX(23)3041)
镇江市重点研发计划项目(NY2023002)。
作者简介
吴志威(1998-),男,硕士研究生,研究方向:食品快速检测与微流控技术,E-mail:2208673845@qq.com;通讯作者:孙宗保(1976-),男,博士,教授,研究方向:食品无损快速检测与农业装备智能化,E-mail:zongbaos@163.com。
