摘要
为揭示滇西南地区野猪肠道微生物组成以及三地不同地理环境对其肠道微生物的影响,研究于2023年9~11月采集临沧、德宏、怒江三地野猪消化道组织,提取DNA后,利用16S rDNA V3~V4区扩增子进行测序和分析。结果表明,从野猪样本中共获得9 761条有效序列,聚类产生8 218个操作分类单元(OTU),分属33个门和554个属,不同地区生活的野猪的粪便菌群结构和功能有所不同。综上,三地野猪的肠道菌群有一定的共性,同时也存在明显的差异。说明不同生活环境会导致野猪肠道微生物菌群差异,生活环境复杂会使得野猪肠道微生物群落更加丰富。
To reveal the composition of the gut microbiota in wild boars from the southwestern region of Yunnan and explore the impact of different geographical environments on their gut microbiota,the study was conducted from September to November 2023,collecting gastrointestinal tract tissues from wild boars in Lincang,Dehong,and Nujiang.After extracting DNA,16S rDNA V3-V4 region amplicon sequencing and analysis were performed.The results showed that a total of 8 218 valid sequences were obtained from the wild boar samples,which were clustered into 8 218 operational taxonomic units(OTUs),belonging to 33 phyla and 554 genera.The Firmicutes and Bacteroidetes were the dominant phyla in all samples.The fecal microbial community structure and function of wild boars living in different regions differed.In summary,the gut microbiota of wild boars from the three regions share certain commonalities,but there are also significant differences.This indicates that different living environments can lead to variations in the gut microbiota of wild boars,and a more complex living environment can result in a richer gut microbial community.
作者
张子蕴
王炳辉
ZHANG Ziyun;WANG Binghui(College of Biodiversity Conservation,Southwest Forestry University,Kunming,Yunnan 650224,China;Kunming Medical University,Kunming,Yunnan 650500,China)
出处
《畜牧兽医杂志》
2025年第3期48-55,共8页
Journal of Animal Science and Veterinary Medicine
关键词
野猪
肠道微生物
滇西南
sus scrofa
gut microbiota
Yunnan Southwest
作者简介
第一作者:张子蕴(1998-),女,硕士研究生,主要从事野生动物研究。E-mail:444537492@qq.com;通信作者:王炳辉,binghuiwang@aliyun.com。
