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改性方法对豆渣不溶性膳食纤维结构、理化性质及功能特性的影响

Effect on the Structure,Physicochemical Properties and Functional Properties of Insoluble Dietary Fiber from Soybean Residue by Modification Methods
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摘要 为探究不同改性方法对豆渣不溶性膳食纤维(Soybean Residue Insoluble Dietary Fiber,SIDF)结构、理化性质及功能特性的影响,采用高速剪切、复合酶解及二者结合的方法对SIDF进行改性处理,通过粒径、傅里叶变换红外光谱(Fourier Transform Infrared Spectroscopy,FTIR)、持水力(Water Holding Capacity,WHC)、持油力(Oil Holding Capacity,OHC)、溶胀力(Swelling Capacity,SC)、阳离子交换能力等指标对SIDF的结构、理化性质及功能特性进行表征。结果表明,改性处理的SIDF粒径显著减小(P<0.05),WHC、OHC、SC显著增强(P<0.05),其中复合改性的SIDF具有最小的粒径,最优的WHC(6.74±0.11 g/g)、OHC(4.22±0.09 g/g)、SC(7.55±0.06 mL/g)。光谱分析表明改性处理后纤维素、半纤维素和木质素发生降解,结晶区发生破坏,其中复合改性处理的样品纤维素等物质降解的最多,结晶区破坏的最为明显。扫描电子显微镜(Scanning Electron Microscope,SEM)结果表明,样品表面出现蜂窝状孔隙结构,其中复合改性的样品孔隙最为密集且最多。改性处理的不溶性膳食纤维具有优异的阳离子交换能力、亚硝酸盐吸附能力等,其中复合改性功能特性最优。综上,高速剪切结合酶解改性处理可以有效改善SIDF的理化性质和功能特性,为其综合利用提供理论依据。 To investigate the effects of different modification methods on the structure,physicochemical and functional properties of soybean residue insoluble dietary fiber(SIDF),high-speed shear,compound enzymatic digestion and the combination of the two methods were used to modify SIDF.The structural,physicochemical and functional properties of SIDF were characterized by particle size,Fourier transform infrared(FTIR)spectroscopy,water holding capacity(WHC),oil holding capacity(OHC),swelling capacity(SC)and cation exchange capacity.Results showed that the particle size of modified SIDF was significantly reduced(P<0.05),and the WHC,OHC,and SC were significantly increased(P<0.05),in which the composite modified SIDF had the smallest particle size and the strongest WHC(6.74±0.11 g/g),OHC(4.22±0.09 g/g),and SC(7.55±0.06 mL/g).Spectral analysis showed that degradation of cellulose,hemicellulose and lignin occurred after the modification treatment and destruction of the crystalline zone,among which cellulose and other substances degraded the most in the composite modification-treated samples,and the destruction of the crystalline zone was the most obvious.Scanning electron microscopy(SEM)results showed that honeycomb pore structure appeared on the surface of the samples,with the composite-modified samples having the densest and most numerous pores.The modified SIDF had excellent cation exchange capacity,nitrite adsorption capacity,etc.,of which the compound modified functional properties were optimal.In summary,high-speed shear combined with enzymatic modification treatment can effectively improve the physicochemical and functional properties of SIDF,providing a theoretical basis for its comprehensive utilization.
作者 张建勋 谢萱 田婷婷 闫世长 齐宝坤 ZHANG Jianxun;XIE Xuan;TIAN Tingting;YAN Shizhang;QI Baokun(College of Food Science,Northeast Agricultural University,Harbin 150030,China)
出处 《食品工业科技》 北大核心 2025年第10期154-162,共9页 Science and Technology of Food Industry
基金 大宗油料油脂释放调控与绿色制油技术(2021YFD2100301-01)。
关键词 豆渣 不溶性膳食纤维 高速剪切 复合酶解 理化性质 功能特性 soybean residue insoluble dietary fiber high-speed shear composite digestion physical and chemical properties functional properties
作者简介 张建勋(2000-),男,硕士研究生,研究方向:粮食、油脂及植物蛋白工程,E-mail:zhangjianxundn@163.com;通信作者:闫世长(1994-),男,博士,教授,研究方向:粮食、油脂及植物蛋白工程,E-mail:yanshizhang@neau.edu.cn;通信作者:齐宝坤(1986-),男,博士,教授,研究方向:粮食、油脂及植物蛋白工程,E-mail:qibaokun22@163.com。
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